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• W2020087178 abstract "Capillary electrophoresis coupled with tandem mass spectrometry was used to indirectly separate and quantify the active metabolite of the anti-human immunodeficiency virus (anti-HIV) didanosine drug. The influence of several parameters (pH and ionic strength of volatile formic acid-ammonia buffer) upon electroosmotic flow, electrophoretic mobility and peak efficiency of several nucleosides (A, dA, ddA, C) has been studied. This paper illustrates the current importance in CE-MS technique as a complementary or substituted method to the known HPLC-radioimmunoassay or HPLC-UV method to measure levels of anti-HIV drugs. The limit of detection for 2',3'-dideoxyadenosine by this method is 2 microg 1(-1) in a formic acid-ammonia buffer (pH 2.5, 10 mM ionic strength). This methodology could be used to perform simultaneous detection of two or more anti-HIV nucleosides, such as stavudine or didanosine in combination therapy." @default.
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• W2020087178 date "2000-10-01" @default.
• W2020087178 modified "2023-09-27" @default.
• W2020087178 title "Determination at ppb level of an anti-human immunodeficiency virus nucleoside drug by capillary electrophoresis–electrospray ionization tandem mass spectrometry" @default.
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• W2020087178 doi "https://doi.org/10.1016/s0021-9673(00)00524-0" @default.
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