Matches in SemOpenAlex for { <https://semopenalex.org/work/W2020108560> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W2020108560 endingPage "3738" @default.
- W2020108560 startingPage "3734" @default.
- W2020108560 abstract "Raltegravir is the first antiretroviral agent to target the human immunodeficiency virus-1 (HIV-1) integrase. It is indicated, in association with other antiretrovirals, in the treatment of acquired immunodeficiency syndrome (AIDS) in antiretroviral treatment-experienced adult patients with viral resistance. To evaluate the feasibility of raltegravir therapeutic drug monitoring, we developed a rapid and specific analytical method for the quantification of raltegravir in human plasma by online sample clean-up liquid chromatography-tandem mass spectrometry (LC-MS/MS).After protein precipitation (with 100 microL of acetonitrile/methanol (50/50)) of 25 microL of plasma, fast online matrix-clean-up was performed using a column switching program. The chromatographic step was optimized to separate raltegravir and its glucuronide metabolite (G-raltegravir). Multiple reaction monitoring (MRM) was used for detection of raltegravir and G-raltegravir. In the absence of G-raltegravir standard, G-raltegravir identification was confirmed by beta-glucuronidase pre-treatment.A total analysis of 3.8 min was needed to separate raltegravir to G-raltegravir. The method was linear between 10 and 3000 ng/mL for raltegravir. Analytical recovery was 94+/-1%. Variation coefficients ranged between 5% and 8.4%. Pre-treatment of plasma from a patient under raltegravir treatment with beta-glucuronidase suppressed G-raltegravir peak.We describe a fast online LC-MS/MS assay that is valid and reliable for the quantification of raltegravir, despite the lack of specificity that could occur in MRM scanning mode experiments." @default.
- W2020108560 created "2016-06-24" @default.
- W2020108560 creator A5013971748 @default.
- W2020108560 creator A5038683026 @default.
- W2020108560 creator A5073886719 @default.
- W2020108560 date "2009-11-01" @default.
- W2020108560 modified "2023-10-10" @default.
- W2020108560 title "Lack of specificity for the analysis of raltegravir using online sample clean-up liquid chromatography–electrospray tandem mass spectrometry" @default.
- W2020108560 cites W2024773741 @default.
- W2020108560 cites W2030815382 @default.
- W2020108560 cites W2039780381 @default.
- W2020108560 cites W2049894560 @default.
- W2020108560 cites W2065576989 @default.
- W2020108560 cites W2075312453 @default.
- W2020108560 cites W2123908462 @default.
- W2020108560 cites W2165447838 @default.
- W2020108560 cites W2322975723 @default.
- W2020108560 doi "https://doi.org/10.1016/j.jchromb.2009.08.031" @default.
- W2020108560 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19744899" @default.
- W2020108560 hasPublicationYear "2009" @default.
- W2020108560 type Work @default.
- W2020108560 sameAs 2020108560 @default.
- W2020108560 citedByCount "12" @default.
- W2020108560 countsByYear W20201085602013 @default.
- W2020108560 countsByYear W20201085602014 @default.
- W2020108560 countsByYear W20201085602015 @default.
- W2020108560 countsByYear W20201085602016 @default.
- W2020108560 countsByYear W20201085602020 @default.
- W2020108560 crossrefType "journal-article" @default.
- W2020108560 hasAuthorship W2020108560A5013971748 @default.
- W2020108560 hasAuthorship W2020108560A5038683026 @default.
- W2020108560 hasAuthorship W2020108560A5073886719 @default.
- W2020108560 hasConcept C112705442 @default.
- W2020108560 hasConcept C142462285 @default.
- W2020108560 hasConcept C159047783 @default.
- W2020108560 hasConcept C162356407 @default.
- W2020108560 hasConcept C185592680 @default.
- W2020108560 hasConcept C2777302000 @default.
- W2020108560 hasConcept C2780272996 @default.
- W2020108560 hasConcept C2780404665 @default.
- W2020108560 hasConcept C2993143319 @default.
- W2020108560 hasConcept C3013748606 @default.
- W2020108560 hasConcept C31827203 @default.
- W2020108560 hasConcept C43617362 @default.
- W2020108560 hasConcept C64489805 @default.
- W2020108560 hasConcept C71043704 @default.
- W2020108560 hasConcept C71924100 @default.
- W2020108560 hasConcept C98274493 @default.
- W2020108560 hasConceptScore W2020108560C112705442 @default.
- W2020108560 hasConceptScore W2020108560C142462285 @default.
- W2020108560 hasConceptScore W2020108560C159047783 @default.
- W2020108560 hasConceptScore W2020108560C162356407 @default.
- W2020108560 hasConceptScore W2020108560C185592680 @default.
- W2020108560 hasConceptScore W2020108560C2777302000 @default.
- W2020108560 hasConceptScore W2020108560C2780272996 @default.
- W2020108560 hasConceptScore W2020108560C2780404665 @default.
- W2020108560 hasConceptScore W2020108560C2993143319 @default.
- W2020108560 hasConceptScore W2020108560C3013748606 @default.
- W2020108560 hasConceptScore W2020108560C31827203 @default.
- W2020108560 hasConceptScore W2020108560C43617362 @default.
- W2020108560 hasConceptScore W2020108560C64489805 @default.
- W2020108560 hasConceptScore W2020108560C71043704 @default.
- W2020108560 hasConceptScore W2020108560C71924100 @default.
- W2020108560 hasConceptScore W2020108560C98274493 @default.
- W2020108560 hasIssue "29" @default.
- W2020108560 hasLocation W20201085601 @default.
- W2020108560 hasLocation W20201085602 @default.
- W2020108560 hasOpenAccess W2020108560 @default.
- W2020108560 hasPrimaryLocation W20201085601 @default.
- W2020108560 hasRelatedWork W1976475396 @default.
- W2020108560 hasRelatedWork W1990380448 @default.
- W2020108560 hasRelatedWork W1994342377 @default.
- W2020108560 hasRelatedWork W2006715152 @default.
- W2020108560 hasRelatedWork W2012666111 @default.
- W2020108560 hasRelatedWork W2106095665 @default.
- W2020108560 hasRelatedWork W2112068078 @default.
- W2020108560 hasRelatedWork W2159933425 @default.
- W2020108560 hasRelatedWork W2163045808 @default.
- W2020108560 hasRelatedWork W2420209185 @default.
- W2020108560 hasVolume "877" @default.
- W2020108560 isParatext "false" @default.
- W2020108560 isRetracted "false" @default.
- W2020108560 magId "2020108560" @default.
- W2020108560 workType "article" @default.