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- W2020110281 abstract "Intact spinach chloroplasts, capable of high rates of photosynthetic CO2 fixation contain 44.3% of the total leaf carbonic anhydrase (carbonate hydro-lyase, EC′ 4.2.1.1) activity. The intact chloroplasts upon increasingly severe osmotic shock treatment lose progressively larger amounts of carbonic anhydrase activity. This loss parallels the removal of the envelope membranes and soluble material from the stroma. Correction for this leakage from the fraction of damaged chloroplasts present in the intact chloroplast preparations yielded a maximal value of 63.0% of the total leaf carbonic anhydrase activity being found in chloroplasts. This carbonic anhydrase activity was associated with the stroma portion of fractionated chloroplasts, and it exhibited a distribution pattern similar to that of ribulose diphosphate carboxylase, a known stroma enzyme. It is suggested that an association between carbonic anhydrase and ribulose diphosphate carboxylase activities occurs in vivo." @default.
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- W2020110281 date "1972-02-01" @default.
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- W2020110281 title "Intracellular distribution of carbonic anhydrase in spinach leaves" @default.
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- W2020110281 doi "https://doi.org/10.1016/0005-2744(72)90255-0" @default.
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