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- W2020112418 abstract "Abstract An experiment was designed to investigate the effect of human fibroblasts, proliferating at low density in culture and accumulating 20:3n-9 and 22:3n-9 into cellular phospholipids, on the concentration of polyunsaturated fatty acids (PUFA) in the culture medium. The cells caused a significant reduction in the total mass of non-esterified fatty acids but did not alter the mass or the fatty acid composition of medium cholesterol ester, triglyceride, or phospholipid. The cells caused a partial depletion of individual saturated fatty acids, monounsaturated fatty acids, and 18:2n-6 but effectively removed all the 20- and 22-carbon polyunsaturated, non-esterified fatty acids. The accumulation of n-9 PUFA in cellular phospholipids occurs under culture conditions where there is a significant depletion of bioavailable PUFA in the culture medium." @default.
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- W2020112418 date "1992-07-01" @default.
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- W2020112418 title "Selective depletion of non-esterified fatty acids in fetal bovine serum-supplemented culture medium by human fibroblasts proliferating in low-density culture" @default.
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- W2020112418 doi "https://doi.org/10.1016/0955-2863(92)90026-f" @default.
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