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• W2020118100 abstract "Mouse sperm nuclei extracted with an ionic detergent and 2 M NaCl retain their overall morphology, but upon subsequent reduction of the protamine disulfides they lose all elements of chromatin structure except the organization of DNA into loop that are anchored to the nuclear matrix. These DNA loops appear as a halo surrounding the nuclear matrix, and nuclei extracted in this manner are, therefore, called nuclear halos. Here, we report that sperm nuclear halos injected into oocytes can form pronuclei, then transform into chromosomes with normal morphology. This suggests that sperm nuclear halos retain all the information necessary for normal chromosomal organization, and that micromanipulation of these extracted sperm nuclei can be accomplished without major DNA damage." @default.
• W2020118100 created "2016-06-24" @default.
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• W2020118100 date "2002-05-21" @default.
• W2020118100 modified "2023-10-09" @default.
• W2020118100 title "Sperm nuclear halos can transform into normal chromosomes after injection into oocytes" @default.
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• W2020118100 doi "https://doi.org/10.1002/mrd.10147" @default.
• W2020118100 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12112607" @default.
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