FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2020124596> ?p ?o ?g. }

• W2020124596 endingPage "86" @default.
• W2020124596 startingPage "75" @default.
• W2020124596 abstract "Accurate measurement of elevated intracellular calcium levels requires indicators with low calcium affinity and high selectivity. We examined fluorescence spectral properties and ionic specificity of three low-affinity, ratiometric indicators structurally related to Fura-2: mag-Fura-2 (furaptra), Fura-2FF, and BTC. The indicators differed in respect to their excitation wavelengths, affinity for Ca2+ (Kd approximately 20 microM, 6 microM and 12 microM respectively) and selectivity over Mg2+ (Kd approximately 2 mM for mag-Fura-2, > 10 mM for Fura-2FF and BTC). Among the tested indicators, BTC was limited by a modest dynamic range upon Ca2+ binding, susceptibility to photodamage, and sensitivity to alterations in pH. All three indicators bound other metal ions including Zn2+, Cd2+ and Gd3+. Interestingly, only in the case of BTC were spectral differences apparent between Ca2+ and other metal ions. For example, the presence of Zn2+ increased BTC fluorescence 6-fold at the Ca2+ isosbestic point, suggesting that this dye may be used as a fluorescent Zn2+ indicator. Fura-2FF has high specificity, wide dynamic range, and low pH sensitivity, and is an optimal low-affinity Ca2+ indicator for most imaging applications. BTC may be useful if experimental conditions require visible wavelength excitation or sensitivity to other metal ions including Zn2+." @default.
• W2020124596 created "2016-06-24" @default.
• W2020124596 creator A5044526549 @default.
• W2020124596 creator A5081691358 @default.
• W2020124596 creator A5082537712 @default.
• W2020124596 date "2000-02-01" @default.
• W2020124596 modified "2023-10-15" @default.
• W2020124596 title "Ionic selectivity of low-affinity ratiometric calcium indicators: mag-Fura-2, Fura-2FF and BTC" @default.
• W2020124596 cites W1499947276 @default.
• W2020124596 cites W1508175871 @default.
• W2020124596 cites W1536233348 @default.
• W2020124596 cites W1601936385 @default.
• W2020124596 cites W1620103507 @default.
• W2020124596 cites W1916482672 @default.
• W2020124596 cites W1963521872 @default.
• W2020124596 cites W1972614641 @default.
• W2020124596 cites W1974450951 @default.
• W2020124596 cites W1975700914 @default.
• W2020124596 cites W1977940662 @default.
• W2020124596 cites W1987163382 @default.
• W2020124596 cites W1987374310 @default.
• W2020124596 cites W1990074560 @default.
• W2020124596 cites W1992637006 @default.
• W2020124596 cites W1992661805 @default.
• W2020124596 cites W1995216096 @default.
• W2020124596 cites W2000119794 @default.
• W2020124596 cites W2003117360 @default.
• W2020124596 cites W2013719399 @default.
• W2020124596 cites W2014074624 @default.
• W2020124596 cites W2015051410 @default.
• W2020124596 cites W2015786690 @default.
• W2020124596 cites W2016181147 @default.
• W2020124596 cites W2017836075 @default.
• W2020124596 cites W2017860747 @default.
• W2020124596 cites W2024616456 @default.
• W2020124596 cites W2026315618 @default.
• W2020124596 cites W2028888912 @default.
• W2020124596 cites W2029623594 @default.
• W2020124596 cites W2032562864 @default.
• W2020124596 cites W2033739116 @default.
• W2020124596 cites W2035897721 @default.
• W2020124596 cites W2038554778 @default.
• W2020124596 cites W2047132570 @default.
• W2020124596 cites W2047735961 @default.
• W2020124596 cites W2048213734 @default.
• W2020124596 cites W2050188036 @default.
• W2020124596 cites W2054566340 @default.
• W2020124596 cites W2056910419 @default.
• W2020124596 cites W2057165491 @default.
• W2020124596 cites W2062272418 @default.
• W2020124596 cites W2069755269 @default.
• W2020124596 cites W2080809219 @default.
• W2020124596 cites W2081577046 @default.
• W2020124596 cites W2081785021 @default.
• W2020124596 cites W2085556812 @default.
• W2020124596 cites W2089386848 @default.
• W2020124596 cites W2107941015 @default.
• W2020124596 cites W2114632806 @default.
• W2020124596 cites W2124005902 @default.
• W2020124596 cites W2127325505 @default.
• W2020124596 cites W2138149556 @default.
• W2020124596 cites W2141255220 @default.
• W2020124596 cites W2141260882 @default.
• W2020124596 cites W2155658105 @default.
• W2020124596 cites W2156715212 @default.
• W2020124596 cites W2159554322 @default.
• W2020124596 cites W2169995490 @default.
• W2020124596 cites W2184699306 @default.
• W2020124596 cites W2337695805 @default.
• W2020124596 cites W2338886577 @default.
• W2020124596 cites W2408281020 @default.
• W2020124596 cites W2413715682 @default.
• W2020124596 cites W2415237386 @default.
• W2020124596 cites W4301760834 @default.
• W2020124596 cites W2001178038 @default.
• W2020124596 doi "https://doi.org/10.1054/ceca.1999.0092" @default.
• W2020124596 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10756974" @default.
• W2020124596 hasPublicationYear "2000" @default.
• W2020124596 type Work @default.
• W2020124596 sameAs 2020124596 @default.
• W2020124596 citedByCount "88" @default.
• W2020124596 countsByYear W20201245962012 @default.
• W2020124596 countsByYear W20201245962013 @default.
• W2020124596 countsByYear W20201245962014 @default.
• W2020124596 countsByYear W20201245962015 @default.
• W2020124596 countsByYear W20201245962017 @default.
• W2020124596 countsByYear W20201245962019 @default.
• W2020124596 countsByYear W20201245962020 @default.
• W2020124596 countsByYear W20201245962021 @default.
• W2020124596 countsByYear W20201245962022 @default.
• W2020124596 countsByYear W20201245962023 @default.
• W2020124596 crossrefType "journal-article" @default.
• W2020124596 hasAuthorship W2020124596A5044526549 @default.
• W2020124596 hasAuthorship W2020124596A5081691358 @default.
• W2020124596 hasAuthorship W2020124596A5082537712 @default.
• W2020124596 hasConcept C113196181 @default.
• W2020124596 hasConcept C118792377 @default.
• W2020124596 hasConcept C121332964 @default.

• next