FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2020129480> ?p ?o ?g. }

• W2020129480 endingPage "6" @default.
• W2020129480 startingPage "1" @default.
• W2020129480 abstract "Classical swine fever virus (CSFV) infection is a severe swine disease, often causing large economic losses. A Pichia pastoris yeast-expressed CSFV glycoprotein E2 (yE2) has been shown to induce a protective immune response against the virus. To improve the expression level of yE2, the first codon of E2 gene, Arg (CGG), which is the least used in P. pastoris, was optimized to the most favorite codon AGA. The yield of E2 protein was remarkably increased in the codon optimized strain (N342). Three truncated E2 subunits encoding the N-terminal 330 (N330), 301 (N301), and 190 (N190) residues, respectively, were also constructed. The immunogenicity of each recombinant E2 subunits was confirmed by immunization of pigs, and all immunized groups demonstrated high neutralizing antibody titers after boost immunization, which lasted for a long period of time. In addition, a monoclonal antibody (MAb), 1B6, specific to yE2, was generated and shown to recognize CSFV-infected cells. A panel of swine sera were tested by peroxidase-conjugated MAb 1B6-based blocking enzyme-linked immunosorbent assay (ELISA) using N330 as coated antigen, and the assay demonstrated high sensitivity and specificity. The recombinant yE2 subunits may provide potential subunit vaccine candidates and useful diagnostic reagents for CSFV with easy manipulation and low cost." @default.
• W2020129480 created "2016-06-24" @default.
• W2020129480 creator A5048401759 @default.
• W2020129480 creator A5054196321 @default.
• W2020129480 creator A5060582674 @default.
• W2020129480 creator A5068484834 @default.
• W2020129480 creator A5079584061 @default.
• W2020129480 creator A5082279982 @default.
• W2020129480 date "2014-03-01" @default.
• W2020129480 modified "2023-09-27" @default.
• W2020129480 title "Enhancing expression of the classical swine fever virus glycoprotein E2 in yeast and its application to a blocking ELISA" @default.
• W2020129480 cites W1521322505 @default.
• W2020129480 cites W1861104203 @default.
• W2020129480 cites W1939748157 @default.
• W2020129480 cites W1964563407 @default.
• W2020129480 cites W1970228759 @default.
• W2020129480 cites W1975624319 @default.
• W2020129480 cites W1981914659 @default.
• W2020129480 cites W1993658549 @default.
• W2020129480 cites W1994975348 @default.
• W2020129480 cites W2012456489 @default.
• W2020129480 cites W2031964159 @default.
• W2020129480 cites W2057655039 @default.
• W2020129480 cites W2061996520 @default.
• W2020129480 cites W2066318233 @default.
• W2020129480 cites W2068507616 @default.
• W2020129480 cites W2071186316 @default.
• W2020129480 cites W2089573206 @default.
• W2020129480 cites W2102093325 @default.
• W2020129480 cites W2108827586 @default.
• W2020129480 cites W2120357935 @default.
• W2020129480 cites W2125112404 @default.
• W2020129480 cites W2165802537 @default.
• W2020129480 cites W2172085583 @default.
• W2020129480 doi "https://doi.org/10.1016/j.jbiotec.2014.01.007" @default.
• W2020129480 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24468422" @default.
• W2020129480 hasPublicationYear "2014" @default.
• W2020129480 type Work @default.
• W2020129480 sameAs 2020129480 @default.
• W2020129480 citedByCount "15" @default.
• W2020129480 countsByYear W20201294802014 @default.
• W2020129480 countsByYear W20201294802015 @default.
• W2020129480 countsByYear W20201294802018 @default.
• W2020129480 countsByYear W20201294802019 @default.
• W2020129480 countsByYear W20201294802020 @default.
• W2020129480 countsByYear W20201294802021 @default.
• W2020129480 countsByYear W20201294802022 @default.
• W2020129480 countsByYear W20201294802023 @default.
• W2020129480 crossrefType "journal-article" @default.
• W2020129480 hasAuthorship W2020129480A5048401759 @default.
• W2020129480 hasAuthorship W2020129480A5054196321 @default.
• W2020129480 hasAuthorship W2020129480A5060582674 @default.
• W2020129480 hasAuthorship W2020129480A5068484834 @default.
• W2020129480 hasAuthorship W2020129480A5079584061 @default.
• W2020129480 hasAuthorship W2020129480A5082279982 @default.
• W2020129480 hasConcept C104317684 @default.
• W2020129480 hasConcept C108625454 @default.
• W2020129480 hasConcept C147483822 @default.
• W2020129480 hasConcept C153911025 @default.
• W2020129480 hasConcept C159047783 @default.
• W2020129480 hasConcept C159654299 @default.
• W2020129480 hasConcept C203014093 @default.
• W2020129480 hasConcept C2522874641 @default.
• W2020129480 hasConcept C2779005923 @default.
• W2020129480 hasConcept C2780801004 @default.
• W2020129480 hasConcept C2780868878 @default.
• W2020129480 hasConcept C2781372317 @default.
• W2020129480 hasConcept C32611913 @default.
• W2020129480 hasConcept C40767141 @default.
• W2020129480 hasConcept C542903549 @default.
• W2020129480 hasConcept C55493867 @default.
• W2020129480 hasConcept C86803240 @default.
• W2020129480 hasConceptScore W2020129480C104317684 @default.
• W2020129480 hasConceptScore W2020129480C108625454 @default.
• W2020129480 hasConceptScore W2020129480C147483822 @default.
• W2020129480 hasConceptScore W2020129480C153911025 @default.
• W2020129480 hasConceptScore W2020129480C159047783 @default.
• W2020129480 hasConceptScore W2020129480C159654299 @default.
• W2020129480 hasConceptScore W2020129480C203014093 @default.
• W2020129480 hasConceptScore W2020129480C2522874641 @default.
• W2020129480 hasConceptScore W2020129480C2779005923 @default.
• W2020129480 hasConceptScore W2020129480C2780801004 @default.
• W2020129480 hasConceptScore W2020129480C2780868878 @default.
• W2020129480 hasConceptScore W2020129480C2781372317 @default.
• W2020129480 hasConceptScore W2020129480C32611913 @default.
• W2020129480 hasConceptScore W2020129480C40767141 @default.
• W2020129480 hasConceptScore W2020129480C542903549 @default.
• W2020129480 hasConceptScore W2020129480C55493867 @default.
• W2020129480 hasConceptScore W2020129480C86803240 @default.
• W2020129480 hasLocation W20201294801 @default.
• W2020129480 hasLocation W20201294802 @default.
• W2020129480 hasOpenAccess W2020129480 @default.
• W2020129480 hasPrimaryLocation W20201294801 @default.
• W2020129480 hasRelatedWork W1024660077 @default.
• W2020129480 hasRelatedWork W1516164909 @default.
• W2020129480 hasRelatedWork W2039236914 @default.
• W2020129480 hasRelatedWork W2092143374 @default.
• W2020129480 hasRelatedWork W2348658746 @default.

• next