Matches in SemOpenAlex for { <https://semopenalex.org/work/W2020155770> ?p ?o ?g. }
- W2020155770 endingPage "4361" @default.
- W2020155770 startingPage "4350" @default.
- W2020155770 abstract "Shotgun proteome analysis of the myxobacterial model strain for secondary metabolite biosynthesis Sorangium cellulosum was performed employing off-line two-dimensional high-pH reversed-phase HPLC x low-pH ion-pair reversed-phase HPLC and dual tandem mass spectrometry with collision-induced dissociation (CID) and electron transfer dissociation (ETD) as complementary fragmentation techniques. Peptide identification using database searching was optimized for ETD fragment spectra to obtain the maximum number of identifications at equivalent false discovery rates (1.0%) in the evaluation of both fragmentation techniques. In the database search of the CID MS/MS data, the mass tolerance was set to the well-established 0.3 Da window, whereas for ETD data, it was widened to 1.1 Da to account for hydrogen-rearrangement in the radical-intermediate of the peptide precursor ion. To achieve a false discovery rate comparable to the CID results, we increased the significance threshold for peptide identification to 0.001 for the ETD data. The ETD based analysis yielded about 74% of all peptides and about 78% of all proteins compared to the CID-method. In the combined data set, 952 proteins of S. cellulosum were confidently identified by at least two peptides per protein, facilitating the study of the function of regulatory proteins in the social myxobacteria and their role in secondary metabolism." @default.
- W2020155770 created "2016-06-24" @default.
- W2020155770 creator A5008574730 @default.
- W2020155770 creator A5008591113 @default.
- W2020155770 creator A5023442853 @default.
- W2020155770 creator A5054358594 @default.
- W2020155770 creator A5074731062 @default.
- W2020155770 creator A5076269700 @default.
- W2020155770 creator A5077818118 @default.
- W2020155770 creator A5090253030 @default.
- W2020155770 date "2009-08-14" @default.
- W2020155770 modified "2023-09-24" @default.
- W2020155770 title "Proteome Analysis of <i>Sorangium cellulosum</i> Employing 2D-HPLC-MS/MS and Improved Database Searching Strategies for CID and ETD Fragment Spectra" @default.
- W2020155770 cites W1495926848 @default.
- W2020155770 cites W1517616120 @default.
- W2020155770 cites W1965714621 @default.
- W2020155770 cites W1968980685 @default.
- W2020155770 cites W1970696723 @default.
- W2020155770 cites W1974196671 @default.
- W2020155770 cites W1979216053 @default.
- W2020155770 cites W1980460714 @default.
- W2020155770 cites W1981171140 @default.
- W2020155770 cites W1981593008 @default.
- W2020155770 cites W1981978551 @default.
- W2020155770 cites W1983191700 @default.
- W2020155770 cites W1984050613 @default.
- W2020155770 cites W1985625499 @default.
- W2020155770 cites W1985956739 @default.
- W2020155770 cites W2003607269 @default.
- W2020155770 cites W2004558299 @default.
- W2020155770 cites W2006015763 @default.
- W2020155770 cites W2007845174 @default.
- W2020155770 cites W2010371815 @default.
- W2020155770 cites W2011419273 @default.
- W2020155770 cites W2018213801 @default.
- W2020155770 cites W2019393077 @default.
- W2020155770 cites W2025325267 @default.
- W2020155770 cites W2026465178 @default.
- W2020155770 cites W2032108825 @default.
- W2020155770 cites W2036264431 @default.
- W2020155770 cites W2042714411 @default.
- W2020155770 cites W2046035907 @default.
- W2020155770 cites W2047275456 @default.
- W2020155770 cites W2048527293 @default.
- W2020155770 cites W2056743521 @default.
- W2020155770 cites W2057731158 @default.
- W2020155770 cites W2063707426 @default.
- W2020155770 cites W2065063131 @default.
- W2020155770 cites W2074824189 @default.
- W2020155770 cites W2082757239 @default.
- W2020155770 cites W2087212852 @default.
- W2020155770 cites W2090806203 @default.
- W2020155770 cites W2108920611 @default.
- W2020155770 cites W2111306975 @default.
- W2020155770 cites W2114931361 @default.
- W2020155770 cites W2121612064 @default.
- W2020155770 cites W2121716393 @default.
- W2020155770 cites W2121974555 @default.
- W2020155770 cites W2127636482 @default.
- W2020155770 cites W2127876423 @default.
- W2020155770 cites W2130706354 @default.
- W2020155770 cites W2131741443 @default.
- W2020155770 cites W2145980150 @default.
- W2020155770 cites W2153180430 @default.
- W2020155770 cites W2154222508 @default.
- W2020155770 cites W2155482447 @default.
- W2020155770 cites W2157115248 @default.
- W2020155770 cites W2162331245 @default.
- W2020155770 cites W2168071329 @default.
- W2020155770 cites W2480365731 @default.
- W2020155770 cites W35024136 @default.
- W2020155770 cites W4293247451 @default.
- W2020155770 doi "https://doi.org/10.1021/pr9004647" @default.
- W2020155770 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19634914" @default.
- W2020155770 hasPublicationYear "2009" @default.
- W2020155770 type Work @default.
- W2020155770 sameAs 2020155770 @default.
- W2020155770 citedByCount "12" @default.
- W2020155770 countsByYear W20201557702012 @default.
- W2020155770 countsByYear W20201557702014 @default.
- W2020155770 countsByYear W20201557702021 @default.
- W2020155770 crossrefType "journal-article" @default.
- W2020155770 hasAuthorship W2020155770A5008574730 @default.
- W2020155770 hasAuthorship W2020155770A5008591113 @default.
- W2020155770 hasAuthorship W2020155770A5023442853 @default.
- W2020155770 hasAuthorship W2020155770A5054358594 @default.
- W2020155770 hasAuthorship W2020155770A5074731062 @default.
- W2020155770 hasAuthorship W2020155770A5076269700 @default.
- W2020155770 hasAuthorship W2020155770A5077818118 @default.
- W2020155770 hasAuthorship W2020155770A5090253030 @default.
- W2020155770 hasConcept C104397665 @default.
- W2020155770 hasConcept C162356407 @default.
- W2020155770 hasConcept C185592680 @default.
- W2020155770 hasConcept C18903297 @default.
- W2020155770 hasConcept C191015642 @default.
- W2020155770 hasConcept C23123220 @default.
- W2020155770 hasConcept C2777109674 @default.
- W2020155770 hasConcept C2777905271 @default.