Matches in SemOpenAlex for { <https://semopenalex.org/work/W2020163483> ?p ?o ?g. }
- W2020163483 endingPage "747" @default.
- W2020163483 startingPage "745" @default.
- W2020163483 abstract "To become insulin independent, patients with type 1 diabetes mellitus require transplantation of at least two donor pancreata because of massive beta-cell loss in the early post-transplantation period. Many studies describing the introduction of new immunosuppressive protocols have shown that this loss is due to not only immunological events but also nonimmunological factors. To test to what extent hypoxia may contribute to early graft loss, we analyzed the occurrence of apoptotic events and the expression of hypoxia-inducible factor 1 (HIF-1), a heterodimeric transcription factor consisting of an oxygen-dependent alpha subunit and a constitutive beta subunit. Histological analysis of human and rat islets revealed nuclear pyknosis as early as 6 h after hypoxic exposure (1% O2). Moreover, immunoreactivity to activated caspase-3 was observed in the core region of isolated human islets. Of note, both of these markers of apoptosis topographically overlap with HIF-1alpha immunoreactivity. HIF-1alpha mRNA was detected in islets from human and rat as well as in several murine beta-cell lines. When exposed to hypoxia, mouse insulinoma cells (MIN6) had an increased HIF-1alpha protein level, whereas its mRNA level did not alter. In conclusion, our data provide convincing evidence that reduced oxygenation is an important cause of beta-cell loss and suggest that HIF-1alpha protein level is an indicator for hypoxic regions undergoing apoptotic cell death. These observations suggest that gene expression under the control of HIF-1 represents a potential therapeutic tool for improving engraftment of transplanted islets." @default.
- W2020163483 created "2016-06-24" @default.
- W2020163483 creator A5006560842 @default.
- W2020163483 creator A5014391609 @default.
- W2020163483 creator A5021419863 @default.
- W2020163483 creator A5026110715 @default.
- W2020163483 creator A5029053733 @default.
- W2020163483 creator A5049963191 @default.
- W2020163483 creator A5062445228 @default.
- W2020163483 creator A5070341110 @default.
- W2020163483 creator A5090247605 @default.
- W2020163483 creator A5091221497 @default.
- W2020163483 date "2002-03-26" @default.
- W2020163483 modified "2023-09-24" @default.
- W2020163483 title "Apoptosis in hypoxic human pancreatic islets correlates with HIF‐1α expression" @default.
- W2020163483 cites W1573536022 @default.
- W2020163483 cites W1578283200 @default.
- W2020163483 cites W1829426772 @default.
- W2020163483 cites W1927872268 @default.
- W2020163483 cites W1937401270 @default.
- W2020163483 cites W1964037605 @default.
- W2020163483 cites W1971286863 @default.
- W2020163483 cites W1987785848 @default.
- W2020163483 cites W1989556220 @default.
- W2020163483 cites W1991843895 @default.
- W2020163483 cites W1997010909 @default.
- W2020163483 cites W1999750787 @default.
- W2020163483 cites W2000624133 @default.
- W2020163483 cites W2001500938 @default.
- W2020163483 cites W2008256610 @default.
- W2020163483 cites W2016730049 @default.
- W2020163483 cites W2025909864 @default.
- W2020163483 cites W2028836414 @default.
- W2020163483 cites W2032662973 @default.
- W2020163483 cites W2037567785 @default.
- W2020163483 cites W2049789970 @default.
- W2020163483 cites W2053386318 @default.
- W2020163483 cites W2054482377 @default.
- W2020163483 cites W2060480804 @default.
- W2020163483 cites W2063242876 @default.
- W2020163483 cites W2068238193 @default.
- W2020163483 cites W2085609942 @default.
- W2020163483 cites W2106710972 @default.
- W2020163483 cites W2109492293 @default.
- W2020163483 cites W2114032717 @default.
- W2020163483 cites W2117074998 @default.
- W2020163483 cites W2124025841 @default.
- W2020163483 cites W2124287509 @default.
- W2020163483 cites W2128095876 @default.
- W2020163483 cites W2137315507 @default.
- W2020163483 cites W2138320519 @default.
- W2020163483 cites W2141063928 @default.
- W2020163483 cites W2148194696 @default.
- W2020163483 cites W2151501496 @default.
- W2020163483 cites W2158844203 @default.
- W2020163483 cites W2160099462 @default.
- W2020163483 cites W2166138837 @default.
- W2020163483 cites W2313829138 @default.
- W2020163483 cites W2321777541 @default.
- W2020163483 cites W2328786770 @default.
- W2020163483 cites W4297900680 @default.
- W2020163483 cites W4321429564 @default.
- W2020163483 cites W94029274 @default.
- W2020163483 doi "https://doi.org/10.1096/fj.01-0403fje" @default.
- W2020163483 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11923216" @default.
- W2020163483 hasPublicationYear "2002" @default.
- W2020163483 type Work @default.
- W2020163483 sameAs 2020163483 @default.
- W2020163483 citedByCount "172" @default.
- W2020163483 countsByYear W20201634832012 @default.
- W2020163483 countsByYear W20201634832013 @default.
- W2020163483 countsByYear W20201634832014 @default.
- W2020163483 countsByYear W20201634832015 @default.
- W2020163483 countsByYear W20201634832016 @default.
- W2020163483 countsByYear W20201634832017 @default.
- W2020163483 countsByYear W20201634832018 @default.
- W2020163483 countsByYear W20201634832019 @default.
- W2020163483 countsByYear W20201634832020 @default.
- W2020163483 countsByYear W20201634832021 @default.
- W2020163483 countsByYear W20201634832022 @default.
- W2020163483 countsByYear W20201634832023 @default.
- W2020163483 crossrefType "journal-article" @default.
- W2020163483 hasAuthorship W2020163483A5006560842 @default.
- W2020163483 hasAuthorship W2020163483A5014391609 @default.
- W2020163483 hasAuthorship W2020163483A5021419863 @default.
- W2020163483 hasAuthorship W2020163483A5026110715 @default.
- W2020163483 hasAuthorship W2020163483A5029053733 @default.
- W2020163483 hasAuthorship W2020163483A5049963191 @default.
- W2020163483 hasAuthorship W2020163483A5062445228 @default.
- W2020163483 hasAuthorship W2020163483A5070341110 @default.
- W2020163483 hasAuthorship W2020163483A5090247605 @default.
- W2020163483 hasAuthorship W2020163483A5091221497 @default.
- W2020163483 hasConcept C104292427 @default.
- W2020163483 hasConcept C104317684 @default.
- W2020163483 hasConcept C126322002 @default.
- W2020163483 hasConcept C134018914 @default.
- W2020163483 hasConcept C160450060 @default.
- W2020163483 hasConcept C165220095 @default.