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• W2020178748 abstract "Les concentrés plaquettaires contaminés sont une cause de septicémie liée á la transfusion. L'incidence varie de 0 á 10 % selon les études. Le but de ce travail est de faire une étude prospective sur la prévalence de la contamination bactérienne des unités plaquettaires standards préparées au Centre régional de transfusion sanguine de Casablanca. Durant 15 mois et demi, 3 458 concentrés de plaquettes standards ont été analysés. Après homogénisation entre le contenu de la poche et le boudin, un cinquième du boudin contenant du concentré plaquettaire a été prélevé chaque jour durant cinq jours et inoculé dans deux tubes contenant 2,5 mL de bouillon tryptocaséine soja. L'un est incubé á 37 °C, l'autre á température ambiante pendant 24 heures. L'identification des germes a été réalisée par des méthodes bactériologiques standards. Vingt-cinq (0,72 %) des 3458 concentrés de plaquettes standards se sont révélés contaminés; 22 (88 %) d'entre eux avaient été stockés trois jours ou plus. Les bactéries retrouvées sont: 14 Staphylococcus coagulase négative, deux Staphylococcus aureus, un Streptococcus alphahémolytique, quatre Bacillus cereus, un Enterobacter cloacae, un Escherichia coli et deux Xanthomonas maltophilia. L'incidence rapportée dans cette série (0,72 %) est supérieure á celle rapportée par la plupart des auteurs (0,02–0,11 %). Cela est dû au fait qu'il s'agit d'une analyse prospective systématique en dehors de tout signe clinique d'appel. Platelet concentrates contaminated with bacteria are a main source of transfusion-associated sepsis. Several studies have reported a very wide incidence (0–10%) of contamination. The aim of this study is to assess the prevalence of bacterial contamination of standard platelet units at the regional blood transfusion center in Casablanca. During 15.5 months, 3,458 platelet concentrates (PCs) were analysed. The plasma from the bag and the tube was homogenised. One-fifth of the tube contents were inoculated every day for five days in two tubes containing 2.5 mL of Tryptic soy broth. One tube was incubated at room temperature and the other at 37° C for 24 hours. The bacteria were identified by standard procedures. Twenty-five (0.72%) of 3,458 platelet bags were contaminated. Twenty-two bacteria (88%) were found in the platelet bags stored three days or more, of which 14 (56%) were Staphylococci coagulase negative, two Staphylococcus aureus, one alpha-haeinolytic Streptococcus, four bacillus cereus, one Enterobacter cloacae, one Escherichia Coli and two Xanthomonas maltophilia. The incidence reported in this series is higher than that reported by most authors." @default.
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• W2020178748 title "Prévalence de la contamination bactérienne dans les unités plaquettaires standards: étude prospective" @default.
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• W2020178748 doi "https://doi.org/10.1016/s1246-7820(00)88946-6" @default.
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