FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2020179035> ?p ?o ?g. }

• W2020179035 endingPage "18181" @default.
• W2020179035 startingPage "18173" @default.
• W2020179035 abstract "Calmodulin (CaM) was shown to be essential for survival of lower eukaryotes by gene deletion experiments. So far, no CaM gene deletion was reported in higher eukaryotes. In vertebrates, CaM is expressed from several genes, which encode an identical protein, making it difficult to generate a model system to study the effect of CaM gene deletion. Here, we present a novel genetic system based on the chicken DT40 cell line, in which the two functional CaM genes were deleted and one allele replaced with a CaM transgene that can be artificially regulated. We show that CaM is essential for survival of vertebrate cells as they die in the absence of CaM expression. Reversal of CaM repression or ectopic expression of HA-tagged CaM rescued the cells. Cells exclusively expressing HA-CaM with impaired individual calcium binding domains as well as HA-CaM lacking the ability to be phosphorylated at residues Tyr(99)/Tyr(138) or trimethylated at Lys(115) survived and grew well. CaM mutated at both Ca(2+) binding sites 3 and 4 as well as at both sites 1 and 2, but to a lesser degree, showed decreased ability to support cell growth. Cells expressing CaM with all calcium binding sites impaired died with kinetics similar to that of cells expressing no CaM. This system offers a unique opportunity to analyze CaM structure-function relationships in vivo without the use of pharmacological inhibitors and to analyze the function of wild type and mutated CaM in modulating the activity of different target systems without interference of endogenous CaM." @default.
• W2020179035 created "2016-06-24" @default.
• W2020179035 creator A5009143476 @default.
• W2020179035 creator A5023659869 @default.
• W2020179035 creator A5031972149 @default.
• W2020179035 creator A5035007833 @default.
• W2020179035 creator A5036377196 @default.
• W2020179035 creator A5045505962 @default.
• W2020179035 creator A5048448458 @default.
• W2020179035 creator A5055611899 @default.
• W2020179035 creator A5063628891 @default.
• W2020179035 creator A5070438350 @default.
• W2020179035 creator A5078423327 @default.
• W2020179035 date "2012-05-01" @default.
• W2020179035 modified "2023-10-18" @default.
• W2020179035 title "Significance of Calcium Binding, Tyrosine Phosphorylation, and Lysine Trimethylation for the Essential Function of Calmodulin in Vertebrate Cells Analyzed in a Novel Gene Replacement System" @default.
• W2020179035 cites W1485297825 @default.
• W2020179035 cites W1553235593 @default.
• W2020179035 cites W1652317765 @default.
• W2020179035 cites W1767701883 @default.
• W2020179035 cites W1963898336 @default.
• W2020179035 cites W1964152667 @default.
• W2020179035 cites W1966416602 @default.
• W2020179035 cites W1967897161 @default.
• W2020179035 cites W1975119602 @default.
• W2020179035 cites W1981090501 @default.
• W2020179035 cites W1987312402 @default.
• W2020179035 cites W1991163286 @default.
• W2020179035 cites W1994720818 @default.
• W2020179035 cites W1995572023 @default.
• W2020179035 cites W2002856612 @default.
• W2020179035 cites W2010464551 @default.
• W2020179035 cites W2012084094 @default.
• W2020179035 cites W2030217314 @default.
• W2020179035 cites W2034454731 @default.
• W2020179035 cites W2040407266 @default.
• W2020179035 cites W2047509412 @default.
• W2020179035 cites W2049367666 @default.
• W2020179035 cites W2052366943 @default.
• W2020179035 cites W2054414022 @default.
• W2020179035 cites W2059020530 @default.
• W2020179035 cites W2063024150 @default.
• W2020179035 cites W2063036476 @default.
• W2020179035 cites W2066093002 @default.
• W2020179035 cites W2077735056 @default.
• W2020179035 cites W2081113123 @default.
• W2020179035 cites W2081136116 @default.
• W2020179035 cites W2084344997 @default.
• W2020179035 cites W2084772014 @default.
• W2020179035 cites W2094173599 @default.
• W2020179035 cites W2101179323 @default.
• W2020179035 cites W2112446189 @default.
• W2020179035 cites W2113708156 @default.
• W2020179035 cites W2131097094 @default.
• W2020179035 cites W2159310265 @default.
• W2020179035 cites W2165104370 @default.
• W2020179035 cites W2165439476 @default.
• W2020179035 cites W4211007332 @default.
• W2020179035 cites W4211204946 @default.
• W2020179035 doi "https://doi.org/10.1074/jbc.m112.339382" @default.
• W2020179035 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3365693" @default.
• W2020179035 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22493455" @default.
• W2020179035 hasPublicationYear "2012" @default.
• W2020179035 type Work @default.
• W2020179035 sameAs 2020179035 @default.
• W2020179035 citedByCount "19" @default.
• W2020179035 countsByYear W20201790352012 @default.
• W2020179035 countsByYear W20201790352013 @default.
• W2020179035 countsByYear W20201790352014 @default.
• W2020179035 countsByYear W20201790352015 @default.
• W2020179035 countsByYear W20201790352016 @default.
• W2020179035 countsByYear W20201790352017 @default.
• W2020179035 countsByYear W20201790352018 @default.
• W2020179035 countsByYear W20201790352019 @default.
• W2020179035 countsByYear W20201790352020 @default.
• W2020179035 countsByYear W20201790352021 @default.
• W2020179035 countsByYear W20201790352022 @default.
• W2020179035 countsByYear W20201790352023 @default.
• W2020179035 crossrefType "journal-article" @default.
• W2020179035 hasAuthorship W2020179035A5009143476 @default.
• W2020179035 hasAuthorship W2020179035A5023659869 @default.
• W2020179035 hasAuthorship W2020179035A5031972149 @default.
• W2020179035 hasAuthorship W2020179035A5035007833 @default.
• W2020179035 hasAuthorship W2020179035A5036377196 @default.
• W2020179035 hasAuthorship W2020179035A5045505962 @default.
• W2020179035 hasAuthorship W2020179035A5048448458 @default.
• W2020179035 hasAuthorship W2020179035A5055611899 @default.
• W2020179035 hasAuthorship W2020179035A5063628891 @default.
• W2020179035 hasAuthorship W2020179035A5070438350 @default.
• W2020179035 hasAuthorship W2020179035A5078423327 @default.
• W2020179035 hasBestOaLocation W20201790351 @default.
• W2020179035 hasConcept C102230213 @default.
• W2020179035 hasConcept C104317684 @default.
• W2020179035 hasConcept C11960822 @default.
• W2020179035 hasConcept C122229402 @default.
• W2020179035 hasConcept C14036430 @default.
• W2020179035 hasConcept C150194340 @default.
• W2020179035 hasConcept C153911025 @default.

• next