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- W2020189449 abstract "Mammal-fish-conserved-sequence 1 (MFCS1) is a highly conserved sequence that acts as a limb-specific cis-acting regulator of Sonic hedgehog (Shh) expression, residing 1 Mb away from the Shh coding sequence in mouse. Using gene-driven screening of an ENU-mutagenized mouse archive, we obtained mice with three new point mutations in MFCS1: M101116, M101117, and M101192. Phenotype analysis revealed that M101116 mice exhibit preaxial polydactyly and ectopic Shh expression at the anterior margin of the limb buds like a previously identified mutant, M100081. In contrast, M101117 and M101192 show no marked abnormalities in limb morphology. Furthermore, transgenic analysis revealed that the M101116 and M100081 sequences drive ectopic reporter gene expression at the anterior margin of the limb bud, in addition to the normal posterior expression. Such ectopic expression was not observed in the embryos carrying a reporter transgene driven by M101117. These results suggest that M101116 and M100081 affect the negative regulatory activity of MFCS1, which suppresses anterior Shh expression in developing limb buds. Thus, this study shows that gene-driven screening for ENU-induced mutations is an effective approach for exploring the function of conserved, noncoding sequences and potential cis-regulatory elements." @default.
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- W2020189449 date "2007-02-01" @default.
- W2020189449 modified "2023-10-14" @default.
- W2020189449 title "A series of ENU-induced single-base substitutions in a long-range cis-element altering Sonic hedgehog expression in the developing mouse limb bud" @default.
- W2020189449 cites W1679252210 @default.
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- W2020189449 cites W1960015501 @default.
- W2020189449 cites W1970810532 @default.
- W2020189449 cites W1971619863 @default.
- W2020189449 cites W1971943177 @default.
- W2020189449 cites W1979641753 @default.
- W2020189449 cites W1982646843 @default.
- W2020189449 cites W1992865216 @default.
- W2020189449 cites W1994966687 @default.
- W2020189449 cites W2008850214 @default.
- W2020189449 cites W2015518796 @default.
- W2020189449 cites W2017204222 @default.
- W2020189449 cites W2017526981 @default.
- W2020189449 cites W2017662103 @default.
- W2020189449 cites W2021485978 @default.
- W2020189449 cites W2022613207 @default.
- W2020189449 cites W2030775870 @default.
- W2020189449 cites W2030879395 @default.
- W2020189449 cites W2049023091 @default.
- W2020189449 cites W2055050547 @default.
- W2020189449 cites W2055792519 @default.
- W2020189449 cites W2057865839 @default.
- W2020189449 cites W2059200013 @default.
- W2020189449 cites W2059532513 @default.
- W2020189449 cites W2064185606 @default.
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- W2020189449 cites W2069160569 @default.
- W2020189449 cites W2077802746 @default.
- W2020189449 cites W2087022717 @default.
- W2020189449 cites W2089962853 @default.
- W2020189449 cites W2096343699 @default.
- W2020189449 cites W2098554686 @default.
- W2020189449 cites W2105180222 @default.
- W2020189449 cites W2106384420 @default.
- W2020189449 cites W2108334325 @default.
- W2020189449 cites W2111256185 @default.
- W2020189449 cites W2114455979 @default.
- W2020189449 cites W2118971690 @default.
- W2020189449 cites W2121157101 @default.
- W2020189449 cites W2121475173 @default.
- W2020189449 cites W2123454904 @default.
- W2020189449 cites W2124070731 @default.
- W2020189449 cites W2127472207 @default.
- W2020189449 cites W2132428980 @default.
- W2020189449 cites W2134581723 @default.
- W2020189449 cites W2143777308 @default.
- W2020189449 cites W2145144631 @default.
- W2020189449 cites W2147627275 @default.
- W2020189449 cites W2147817030 @default.
- W2020189449 cites W2153145187 @default.
- W2020189449 cites W2157461735 @default.
- W2020189449 cites W2158919523 @default.
- W2020189449 cites W2159755919 @default.
- W2020189449 cites W2160220594 @default.
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- W2020189449 cites W2162307732 @default.
- W2020189449 cites W2163838718 @default.
- W2020189449 cites W2171516311 @default.
- W2020189449 cites W2181541017 @default.
- W2020189449 cites W2187129279 @default.
- W2020189449 cites W4248591220 @default.
- W2020189449 cites W4252593641 @default.
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- W2020189449 doi "https://doi.org/10.1016/j.ygeno.2006.09.005" @default.
- W2020189449 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17049204" @default.
- W2020189449 hasPublicationYear "2007" @default.
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