FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2020190010> ?p ?o ?g. }

• W2020190010 endingPage "944" @default.
• W2020190010 startingPage "935" @default.
• W2020190010 abstract "In the central nervous system, synaptic levels of the monoamine neurotransmitter serotonin are mainly controlled by the serotonin transporter (SERT), and drugs used in the treatment of various psychiatric diseases have SERT as primary target. SERT is a phosphoprotein that undergoes phosphorylation/dephosphorylation during transporter regulation by multiple pathways. In particular, activation and/or inhibition of kinases including PKC, PKG, p38MAPK, and CaMKII modulate SERT function and trafficking. The molecular mechanisms by which kinase activity is linked to SERT regulation are poorly understood, including the identity of specific phosphorylated residues. To elucidate SERT phosphorylation sites, we have generated peptides corresponding to the entire intracellular region of human SERT and performed in vitro phosphorylation assays with a panel of kinases suggested to be involved in SERT regulation or for which canonical phosphorylation sites are predicted. Peptide analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to identify and quantify site-specific phosphorylation. Five residues located in the N- and C-termini and in intracellular loop 1 and 2 were identified as phosphorylation sites; Ser149, Ser277, and Thr603 for PKC, Ser13 for CaMKII, and Thr616 for p38MAPK. Possible regulatory roles of these potential phosphoacceptors for SERT function and surface expression were investigated using phospho-mimicking and phosphodeficient mutations, coexpression of constitutively active kinases and pharmacological kinase induction in a heterologous expression system. Our results suggest that Ser277 is involved in an initial phase of PKC-mediated down-regulation of SERT. The five identified sites can guide future studies of direct links between SERT phosphorylation and regulatory processes." @default.
• W2020190010 created "2016-06-24" @default.
• W2020190010 creator A5043792916 @default.
• W2020190010 creator A5060992391 @default.
• W2020190010 creator A5062853847 @default.
• W2020190010 date "2014-02-03" @default.
• W2020190010 modified "2023-10-02" @default.
• W2020190010 title "Characterization of Intracellular Regions in the Human Serotonin Transporter for Phosphorylation Sites" @default.
• W2020190010 cites W1489638598 @default.
• W2020190010 cites W1570313229 @default.
• W2020190010 cites W1604642620 @default.
• W2020190010 cites W1848879777 @default.
• W2020190010 cites W1850214287 @default.
• W2020190010 cites W1911240071 @default.
• W2020190010 cites W1965602650 @default.
• W2020190010 cites W1968629260 @default.
• W2020190010 cites W1970124769 @default.
• W2020190010 cites W1970870670 @default.
• W2020190010 cites W1975102846 @default.
• W2020190010 cites W1988760898 @default.
• W2020190010 cites W1992091012 @default.
• W2020190010 cites W1994195073 @default.
• W2020190010 cites W1994458658 @default.
• W2020190010 cites W1996120254 @default.
• W2020190010 cites W1997925369 @default.
• W2020190010 cites W2008563232 @default.
• W2020190010 cites W2010248207 @default.
• W2020190010 cites W2013844154 @default.
• W2020190010 cites W2015068634 @default.
• W2020190010 cites W2021521177 @default.
• W2020190010 cites W2027164618 @default.
• W2020190010 cites W2027980594 @default.
• W2020190010 cites W2033312676 @default.
• W2020190010 cites W2043794217 @default.
• W2020190010 cites W2052322593 @default.
• W2020190010 cites W2053896453 @default.
• W2020190010 cites W2055751498 @default.
• W2020190010 cites W2055924599 @default.
• W2020190010 cites W2059571537 @default.
• W2020190010 cites W2065963618 @default.
• W2020190010 cites W2073179024 @default.
• W2020190010 cites W2083246985 @default.
• W2020190010 cites W2087803534 @default.
• W2020190010 cites W2104244040 @default.
• W2020190010 cites W2118741643 @default.
• W2020190010 cites W2122565898 @default.
• W2020190010 cites W2124241220 @default.
• W2020190010 cites W2124293356 @default.
• W2020190010 cites W2126063965 @default.
• W2020190010 cites W2128763007 @default.
• W2020190010 cites W2133500918 @default.
• W2020190010 cites W2133944605 @default.
• W2020190010 cites W2146092354 @default.
• W2020190010 cites W2160253297 @default.
• W2020190010 cites W2165923048 @default.
• W2020190010 cites W3021613818 @default.
• W2020190010 cites W4246091758 @default.
• W2020190010 doi "https://doi.org/10.1021/cb4007198" @default.
• W2020190010 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24450286" @default.
• W2020190010 hasPublicationYear "2014" @default.
• W2020190010 type Work @default.
• W2020190010 sameAs 2020190010 @default.
• W2020190010 citedByCount "22" @default.
• W2020190010 countsByYear W20201900102015 @default.
• W2020190010 countsByYear W20201900102016 @default.
• W2020190010 countsByYear W20201900102017 @default.
• W2020190010 countsByYear W20201900102018 @default.
• W2020190010 countsByYear W20201900102019 @default.
• W2020190010 countsByYear W20201900102020 @default.
• W2020190010 countsByYear W20201900102021 @default.
• W2020190010 countsByYear W20201900102022 @default.
• W2020190010 crossrefType "journal-article" @default.
• W2020190010 hasAuthorship W2020190010A5043792916 @default.
• W2020190010 hasAuthorship W2020190010A5060992391 @default.
• W2020190010 hasAuthorship W2020190010A5062853847 @default.
• W2020190010 hasConcept C11960822 @default.
• W2020190010 hasConcept C170493617 @default.
• W2020190010 hasConcept C178666793 @default.
• W2020190010 hasConcept C181912034 @default.
• W2020190010 hasConcept C184235292 @default.
• W2020190010 hasConcept C195794163 @default.
• W2020190010 hasConcept C2775864247 @default.
• W2020190010 hasConcept C2777061661 @default.
• W2020190010 hasConcept C2778897192 @default.
• W2020190010 hasConcept C2908747647 @default.
• W2020190010 hasConcept C55493867 @default.
• W2020190010 hasConcept C86803240 @default.
• W2020190010 hasConcept C87325107 @default.
• W2020190010 hasConcept C95444343 @default.
• W2020190010 hasConcept C97029542 @default.
• W2020190010 hasConceptScore W2020190010C11960822 @default.
• W2020190010 hasConceptScore W2020190010C170493617 @default.
• W2020190010 hasConceptScore W2020190010C178666793 @default.
• W2020190010 hasConceptScore W2020190010C181912034 @default.
• W2020190010 hasConceptScore W2020190010C184235292 @default.
• W2020190010 hasConceptScore W2020190010C195794163 @default.
• W2020190010 hasConceptScore W2020190010C2775864247 @default.
• W2020190010 hasConceptScore W2020190010C2777061661 @default.

• next