FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2020193432> ?p ?o ?g. }

• W2020193432 endingPage "698" @default.
• W2020193432 startingPage "S" @default.
• W2020193432 abstract "Introduction Since their invention 14 years ago, by Chinese pharmacist Hon Lik, the electronic cigarette (e-cigarette) has known increasing success, experiencing a particular surge in popularity post 2010. Their usage has allowed for reduced tobacco consumption which can be attributed to a reduction in health concerns such as Crohn's Disease. However, it is important to clarify whether or not the e-cigarette may also present a number of health risks. Previous work carried out by our team, examining the effect of exposure to cigarette smoke on immune cells taken from patients with inflammatory bowel disease (IBD), confirmed the dual effect on IBD inflammatory response after tobacco exposure [1]. In this context, examining the toxicity of products present in the smoke from traditional cigarettes versus e-cigarettes, presented an interesting topic. The aim of this study was to compare the impact of the e-cigarette with that of tobacco on inflammatory response in an epithelial intestinal cell culture model. Materials and Methods The intestinal inflammatory response was evaluated using a human intestinal epithelial cell line model (HT29), transfected with bacterial LPS receptor, MD-2. This model presents an exaggerated inflammatory response to LPS, a characteristic which presented in Crohn's disease. Release of interleukin (IL)-8, a marker of inflammation, was measured by ELISA for control cells or those stimulated by LPS and after exposure to either traditional or e-cigarettes smoke extracted in parallel using a machine which mimics the act of smoking. The concentration of nicotine was equivalent in both extracts (traditional and e-cigarette). Controls were performed in the presence of smoke containing only the e-cigarette solvent, propylene glycol. Toxicity was evaluated by tetrazolium salt MTT toxicity test. Results Inflammatory response was greatly elevated in cells exposed to traditional cigarette (tc) smoke, as measured by IL-8 release (pg/mg protein). In contrast, cells exposed to e-cigarette smoke (ec) showed inflammatory response comparable to control cells (sol) and significantly lower than those treated with traditional cigarette (tc) smoke extracts. IL-8 release (pg/mg protein) was observed as follows: for non-LPS stimulated (control) cells; 79.6±10.1 (controls) vs 175.2±16.6 (tc) and 68.6±4 (ec) vs 77.7±10.7 (sol) and for cells treated with 10 μg/ml LPS; 1507.8 ±228.6 (controls) vs 2684.7±632.1 (tc) and 1287,5±235 (ec) vs 1570,9±224,8 (sol). Conclusion Our results suggest that the intestinal epithelium inflammatory response is not altered by exposure to smoke from e-cigarettes. Further research into the consequences of e-cigarettes on immune cells will aid to complement these encouraging results and serve to refine our current knowledge on the dual effect of tobacco in Crohn's disease and ulcerative colitis." @default.
• W2020193432 created "2016-06-24" @default.
• W2020193432 creator A5001660061 @default.
• W2020193432 creator A5021752739 @default.
• W2020193432 creator A5049096801 @default.
• W2020193432 creator A5058946827 @default.
• W2020193432 creator A5074099993 @default.
• W2020193432 date "2015-04-01" @default.
• W2020193432 modified "2023-10-16" @default.
• W2020193432 title "Mo1737 Granulo-Monocytes Apheresis Induce TGFβ1 Modulation in Neutrophils of Patients Suffering From Ulcerative Colitis: A Possible Role of Soluble HLA-I Molecules" @default.
• W2020193432 doi "https://doi.org/10.1016/s0016-5085(15)32367-2" @default.
• W2020193432 hasPublicationYear "2015" @default.
• W2020193432 type Work @default.
• W2020193432 sameAs 2020193432 @default.
• W2020193432 citedByCount "0" @default.
• W2020193432 crossrefType "journal-article" @default.
• W2020193432 hasAuthorship W2020193432A5001660061 @default.
• W2020193432 hasAuthorship W2020193432A5021752739 @default.
• W2020193432 hasAuthorship W2020193432A5049096801 @default.
• W2020193432 hasAuthorship W2020193432A5058946827 @default.
• W2020193432 hasAuthorship W2020193432A5074099993 @default.
• W2020193432 hasConcept C118131993 @default.
• W2020193432 hasConcept C126322002 @default.
• W2020193432 hasConcept C185592680 @default.
• W2020193432 hasConcept C203014093 @default.
• W2020193432 hasConcept C2776738589 @default.
• W2020193432 hasConcept C2779134260 @default.
• W2020193432 hasConcept C2780479503 @default.
• W2020193432 hasConcept C502942594 @default.
• W2020193432 hasConcept C71924100 @default.
• W2020193432 hasConcept C89560881 @default.
• W2020193432 hasConceptScore W2020193432C118131993 @default.
• W2020193432 hasConceptScore W2020193432C126322002 @default.
• W2020193432 hasConceptScore W2020193432C185592680 @default.
• W2020193432 hasConceptScore W2020193432C203014093 @default.
• W2020193432 hasConceptScore W2020193432C2776738589 @default.
• W2020193432 hasConceptScore W2020193432C2779134260 @default.
• W2020193432 hasConceptScore W2020193432C2780479503 @default.
• W2020193432 hasConceptScore W2020193432C502942594 @default.
• W2020193432 hasConceptScore W2020193432C71924100 @default.
• W2020193432 hasConceptScore W2020193432C89560881 @default.
• W2020193432 hasIssue "4" @default.
• W2020193432 hasLocation W20201934321 @default.
• W2020193432 hasOpenAccess W2020193432 @default.
• W2020193432 hasPrimaryLocation W20201934321 @default.
• W2020193432 hasRelatedWork W2025048290 @default.
• W2020193432 hasRelatedWork W2086156447 @default.
• W2020193432 hasRelatedWork W2375240245 @default.
• W2020193432 hasRelatedWork W2464962541 @default.
• W2020193432 hasRelatedWork W2475104063 @default.
• W2020193432 hasRelatedWork W2748952813 @default.
• W2020193432 hasRelatedWork W2896381073 @default.
• W2020193432 hasRelatedWork W2899084033 @default.
• W2020193432 hasRelatedWork W3147501336 @default.
• W2020193432 hasRelatedWork W4210249856 @default.
• W2020193432 hasVolume "148" @default.
• W2020193432 isParatext "false" @default.
• W2020193432 isRetracted "false" @default.
• W2020193432 magId "2020193432" @default.
• W2020193432 workType "article" @default.