Matches in SemOpenAlex for { <https://semopenalex.org/work/W2020202354> ?p ?o ?g. }
- W2020202354 endingPage "1164" @default.
- W2020202354 startingPage "1164" @default.
- W2020202354 abstract "Purpose.: To assess oxysterol-induced mitochondrial DNA (mtDNA) damage and mitochondrial dysfunction in cultured human retinal pigment epithelial cells (ARPE- 19). Methods.: ARPE-19 cultures were exposed for 6 and 24 hours to 40 μg/mL 7-ketocholesterol (7kCh), and total DNA was extracted. Long-extension polymerase chain reaction was performed to amplify the full-length mtDNA genome. The products were separated by electrophoresis on a 0.8% agarose gel stained with ethidium bromide. Superoxide and reactive oxygen/nitrogen species (ROS/RNS; hydrogen peroxide, peroxynitrite anions, and peroxyl radicals) were measured with an amine-reactive green-dye assay and 2`,7`-dicholorodihydrofluorescein diacetate (H2DCFDA) dye assay, respectively. The changes in mitochondrial membrane potential (ΔΨm) were measured with a cationic (green) dye assay. Western blot analysis was used to assess porins, a structural protein of the mitochondrial membranes. Results.: The 7kCh-treated cultures showed significant increase in ROS/RNS production (P < 0.001) compared with untreated controls, but the superoxide levels were unchanged. The 7kCh-treated ARPE-19 cultures had diminished levels of the full-length 16.2-kb mtDNA band, a 2.2-fold decrease of the ΔΨm compared with control cultures (P < 0.001), and decreased levels of porins. Conclusions.: 7kCh causes significant damage to the full-length intact mtDNA and mitochondrial dysfunction in ARPE-19 cells. These observations suggest that the mitochondria and its DNA may be targets for oxysterol-induced oxidative stress and may play a role in the pathogenesis of retinal diseases." @default.
- W2020202354 created "2016-06-24" @default.
- W2020202354 creator A5013287462 @default.
- W2020202354 creator A5014763857 @default.
- W2020202354 creator A5031577547 @default.
- W2020202354 creator A5037034652 @default.
- W2020202354 creator A5037039697 @default.
- W2020202354 creator A5075683156 @default.
- W2020202354 creator A5078489850 @default.
- W2020202354 creator A5086598174 @default.
- W2020202354 creator A5090570484 @default.
- W2020202354 date "2010-02-01" @default.
- W2020202354 modified "2023-09-23" @default.
- W2020202354 title "Mitochondrial DNA Damage Induced by 7-Ketocholesterol in Human Retinal Pigment Epithelial Cells In Vitro" @default.
- W2020202354 cites W1479899908 @default.
- W2020202354 cites W1565600091 @default.
- W2020202354 cites W158938192 @default.
- W2020202354 cites W1941964179 @default.
- W2020202354 cites W1965022343 @default.
- W2020202354 cites W1965259073 @default.
- W2020202354 cites W1965997548 @default.
- W2020202354 cites W1966766618 @default.
- W2020202354 cites W1967683528 @default.
- W2020202354 cites W1968980411 @default.
- W2020202354 cites W1970385641 @default.
- W2020202354 cites W1973078232 @default.
- W2020202354 cites W1973156968 @default.
- W2020202354 cites W1975369096 @default.
- W2020202354 cites W1976014159 @default.
- W2020202354 cites W1977670584 @default.
- W2020202354 cites W1992124035 @default.
- W2020202354 cites W1992788147 @default.
- W2020202354 cites W1994079695 @default.
- W2020202354 cites W1996881587 @default.
- W2020202354 cites W2012038670 @default.
- W2020202354 cites W2012215983 @default.
- W2020202354 cites W2012306090 @default.
- W2020202354 cites W2024729125 @default.
- W2020202354 cites W2026072968 @default.
- W2020202354 cites W2037895894 @default.
- W2020202354 cites W2040417704 @default.
- W2020202354 cites W2041767939 @default.
- W2020202354 cites W2043997604 @default.
- W2020202354 cites W2044012773 @default.
- W2020202354 cites W2044494908 @default.
- W2020202354 cites W2047393488 @default.
- W2020202354 cites W2053778733 @default.
- W2020202354 cites W2055541535 @default.
- W2020202354 cites W2058381006 @default.
- W2020202354 cites W2060815384 @default.
- W2020202354 cites W2066016132 @default.
- W2020202354 cites W2066062165 @default.
- W2020202354 cites W2068297435 @default.
- W2020202354 cites W2068717970 @default.
- W2020202354 cites W2069014351 @default.
- W2020202354 cites W2070123605 @default.
- W2020202354 cites W2074966907 @default.
- W2020202354 cites W2076477831 @default.
- W2020202354 cites W2077366389 @default.
- W2020202354 cites W2087292294 @default.
- W2020202354 cites W2087678136 @default.
- W2020202354 cites W2087892120 @default.
- W2020202354 cites W2088084771 @default.
- W2020202354 cites W2092976867 @default.
- W2020202354 cites W2092990740 @default.
- W2020202354 cites W2094956499 @default.
- W2020202354 cites W2095058526 @default.
- W2020202354 cites W2099380745 @default.
- W2020202354 cites W2105902252 @default.
- W2020202354 cites W2109291482 @default.
- W2020202354 cites W2112694631 @default.
- W2020202354 cites W2121176183 @default.
- W2020202354 cites W2126773395 @default.
- W2020202354 cites W2128222819 @default.
- W2020202354 cites W2134848936 @default.
- W2020202354 cites W2137792435 @default.
- W2020202354 cites W2138268741 @default.
- W2020202354 cites W2147031368 @default.
- W2020202354 cites W2163417125 @default.
- W2020202354 cites W2166550315 @default.
- W2020202354 cites W2336578011 @default.
- W2020202354 cites W3022864527 @default.
- W2020202354 cites W30467198 @default.
- W2020202354 cites W31102387 @default.
- W2020202354 cites W81185256 @default.
- W2020202354 cites W96569355 @default.
- W2020202354 doi "https://doi.org/10.1167/iovs.09-3443" @default.
- W2020202354 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19834037" @default.
- W2020202354 hasPublicationYear "2010" @default.
- W2020202354 type Work @default.
- W2020202354 sameAs 2020202354 @default.
- W2020202354 citedByCount "31" @default.
- W2020202354 countsByYear W20202023542012 @default.
- W2020202354 countsByYear W20202023542013 @default.
- W2020202354 countsByYear W20202023542014 @default.
- W2020202354 countsByYear W20202023542016 @default.
- W2020202354 countsByYear W20202023542017 @default.
- W2020202354 countsByYear W20202023542018 @default.