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- W2020242875 abstract "The glutamate-producing bacterium, Corynebacterium glutamicum is known to possess two anaplerotic enzymes: pyruvate carboxylase (Pc) and phosphoenolpyruvate carboxylase (PEPc). In vitro, this latter enzyme appeared to be inhibited by different glutamic acid salts, whereas ammonium-glutamate had no influence on Pc activity. To investigate the in vivo relevance of PEPc activity inhibition, the intracellular concentration of glutamate was determined throughout the glutamate-producing process. The intracellular concentration was then shown to be sufficient to induce a dramatic inhibition of PEPc activity during the process. As a consequence, intracellular accumulation of glutamate could be at least partially responsible for the weak participation of PEPc within the anaplerosis activity in amino-acid-producing strains of C. glutamicum." @default.
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- W2020242875 date "2004-05-01" @default.
- W2020242875 modified "2023-09-27" @default.
- W2020242875 title "Glutamate as an inhibitor of phosphoenolpyruvate carboxylase activity in Corynebacterium glutamicum" @default.
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- W2020242875 doi "https://doi.org/10.1007/s10295-004-0137-6" @default.
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