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• W2020260839 abstract "Magnetic immuno PCR assay (MIPA) was developed for the rapid detection of leptospires excreted in urine samples (n = 59) collected from 35 experimentally infected cattle. The immunomagnetic separation of leptospires from inhibitors in frozen formalin fixed bovine urine prior to PCR detection resulted in a marked improvement on previous detection methods. MIPA is a rapid 5 step protocol requiring 70 mins preparation time prior to amplification, which consistently detects 101 organisms. MIPA detected 76% (38/50) of culture positive urines and in addition three urines that were culture negative were shown to be positive by this method of detection. Consequently we conclude that whilst MIPA is an improvement on previously published PCR detection methods, the culture of the organism is still the standard against which other detection methods have to be compared." @default.
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• W2020260839 date "1997-05-01" @default.
• W2020260839 modified "2023-09-26" @default.
• W2020260839 title "Magnetic immuno capture PCR assay (MIPA): Detection of Leptospira borgpetersenii serovar hardjo" @default.
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• W2020260839 doi "https://doi.org/10.1016/s0378-1135(96)01351-x" @default.
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