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- W2020280811 abstract "Production of the nuclear polyhedrosis viruses (NPV) ofSpodoptera exiguaandS. exemptawas studied experimentally to determine the conditions for optimumin vivoproduction in the homologous hosts. Larvae ofS. exiguaandS. exemptawere separately reared in individual cells on artificial diet which had been sprayed with a range of concentrations equivalent to 1 × 102to 1 × 107occlusion bodies (OB)/cell. The maximum yield obtained for individual larvae was 1.40 ± 0.28 × 109OB (±SE) forS. exiguadosed as 5–15 mg larvae with 1 × 106OB/cell and harvested after 5 days' incubation and 3.29 ± 0.46 × 109OB forS. exemptadosed as 31–50 mg larvae also with 1 × 106OB/cell and harvested after 7 days' incubation. Optimized yields per diet cell were 8.02 ± 2.40 × 108OB forS. exiguaand 2.30 ± 0.59 × 109OB forS. exempta.LT50values fell with dose from 10.3 days at 1 × 104OB/cell to 5.5 days at 1 × 107OB/cell forS. exempta.Postharvest incubation led to slight OB yield increases forS. exiguabut not forS. exemptaand to a large increase in bacterial contamination. Low to moderate OB doses increased larval weight relative to that of control larvae in both species." @default.
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- W2020280811 date "1997-07-01" @default.
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- W2020280811 title "The Optimization ofin VivoNuclear Polyhedrosis Virus Production inSpodoptera exempta(Walker) andSpodoptera exigua(Hübner)" @default.
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