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• W2020288201 abstract "Förster resonance energy transfer (FRET) is a powerful tool in studying biomolecular interactions. Intrinsically fluorescent lanthanide chelates are increasingly used as FRET donors due to their long emission lifetime that enables the use of time resolution. Fluorescent proteins, on the other hand, owe their popularity to the intrinsic luminescent properties, facilitating their use as fusion proteins. In this investigation, two energy transfer pairs, terbium(III) chelate with green fluorescent protein (GFP) and europium(III) chelate with yellow fluorescent protein (YFP), were studied by expressing the fluorescent protein acceptor as a fusion protein together with Rab21 GTPase. GTP-conjugated lanthanide chelates were used as donor conjugates. In contrast to conventional FRET observed with the Tb3+−GFP pair, a phenomenon called nonoverlapping FRET (nFRET) was observed with the Eu3+−YFP pair. In nFRET, the sensitized emission of the acceptor was measured at shorter wavelength than where the emission of the donor was observed. Regardless of the lower signal levels, nFRET resulted in a substantially higher signal-to-background ratio. Conventional FRET from sensitized acceptor yielded a single apparent fluorescence emission lifetime, while with nFRET two lifetimes were observed. The lanthanide chelates together with fluorescent proteins enable a straightforward and sensitive assay technology in nFRET applications." @default.
• W2020288201 created "2016-06-24" @default.
• W2020288201 creator A5032517066 @default.
• W2020288201 creator A5040921944 @default.
• W2020288201 creator A5084076358 @default.
• W2020288201 date "2009-05-13" @default.
• W2020288201 modified "2023-09-27" @default.
• W2020288201 title "Resonance Energy Transfer from Lanthanide Chelates to Overlapping and Nonoverlapping Fluorescent Protein Acceptors" @default.
• W2020288201 cites W1580530266 @default.
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• W2020288201 doi "https://doi.org/10.1021/ac9005793" @default.
• W2020288201 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19438245" @default.
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