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• W2020296413 abstract "A method was developed to functionalize biomedical metals with liposomes. The novelty of the method includes the plasma-functionalization of the metal surface with proper chemical groups to be used as anchor sites for the covalent immobilization of the liposomes. Stainless steel (SS-316) disks were processed in radiofrequency glow discharges fed with vapors of acrylic acid to coat them with thin adherent films characterized by surface carboxylic groups, where liposomes were covalently bound through the formation of amide bonds. For this, liposomes decorated with polyethylene glycol molecules bearing terminal amine-groups were prepared. After ensuring that the liposomes remain intact, under the conditions applying for immobilization; different attachment conditions were evaluated (incubation time, concentration of liposome dispersion) for optimization of the technique. Immobilization of calcein-entrapping liposomes was evaluated by monitoring the percent of calcein attached on the surfaces. Best results were obtained when liposome dispersions with 5 mg/ml (liposomal lipid) concentration were incubated on each disk for 24 h at 37 °C. The method is proposed for developing drug-eluting biomedical materials or devices by using liposomes that have appropriate membrane compositions and are loaded with drugs or other bioactive agents." @default.
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• W2020296413 date "2011-05-01" @default.
• W2020296413 modified "2023-10-18" @default.
• W2020296413 title "Covalent immobilization of liposomes on plasma functionalized metallic surfaces" @default.
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• W2020296413 doi "https://doi.org/10.1016/j.colsurfb.2011.01.002" @default.
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