FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2020350080> ?p ?o ?g. }

• W2020350080 abstract "Renal tubular epithelial cells of proximal and distal origin differ markedly in their physiological functions. Therefore, we hypothesized that they also differ in their capacity to undergo epithelial to mesenchymal alterations.We used cultures of freshly isolated primary human tubular cells. To distinguish cells of different tubular origin we took advantage of the fact that human proximal epithelial cells uniquely express N-cadherin instead of E-cadherin as major cell-cell adhesion molecule. To provoke mesenchymal alteration we treated these cocultures with TGF-β for up to 6 days. Within this time period, the morphology of distal tubular cells was barely altered. In contrast to tubular cell lines, E-cadherin was not down-regulated by TGF-β, even though TGF-β signal transduction was initiated as demonstrated by nuclear localization of Smad2/3. Analysis of transcription factors and miRNAs possibly involved in E-cadherin regulation revealed high levels of miRNAs of the miR200-family, which may contribute to the stability of E-cadherin expression in human distal tubular epithelial cells. By contrast, proximal tubular epithelial cells altered their phenotype when treated with TGF-β. They became elongated and formed three-dimensional structures. Rho-kinases were identified as modulators of TGF-β-induced morphological alterations. Non-specific inhibition of Rho-kinases resulted in stabilization of the epithelial phenotype, while partial effects were observed upon downregulation of Rho-kinase isoforms ROCK1 and ROCK2. The distinct reactivity of proximal and distal cells was retained when the cells were cultured as polarized cells.Interference with Rho-kinase signaling provides a target to counteract TGF-β-mediated mesenchymal alterations of epithelial cells, particularly in proximal tubular epithelial cells. Furthermore, primary distal tubular cells differed from cell lines by their high phenotypic stability which included constant expression of E-cadherin. Our cell culture system of primary epithelial cells is thus suitable to understand and modulate cellular remodeling processes of distinct tubular cells relevant for human renal disease." @default.
• W2020350080 created "2016-06-24" @default.
• W2020350080 creator A5006529578 @default.
• W2020350080 creator A5025632358 @default.
• W2020350080 creator A5042634912 @default.
• W2020350080 creator A5045660349 @default.
• W2020350080 creator A5063407939 @default.
• W2020350080 creator A5073558224 @default.
• W2020350080 date "2012-01-01" @default.
• W2020350080 modified "2023-09-25" @default.
• W2020350080 title "Distinct mesenchymal alterations in N-cadherin and E-cadherin positive primary renal epithelial cells." @default.
• W2020350080 cites W1568555760 @default.
• W2020350080 cites W1597258706 @default.
• W2020350080 cites W1835009115 @default.
• W2020350080 cites W1850950958 @default.
• W2020350080 cites W1912639232 @default.
• W2020350080 cites W1964081206 @default.
• W2020350080 cites W1969109536 @default.
• W2020350080 cites W1969564302 @default.
• W2020350080 cites W1970604167 @default.
• W2020350080 cites W1972363153 @default.
• W2020350080 cites W1974150901 @default.
• W2020350080 cites W1974383369 @default.
• W2020350080 cites W1976425440 @default.
• W2020350080 cites W1980566259 @default.
• W2020350080 cites W1984870208 @default.
• W2020350080 cites W1984939681 @default.
• W2020350080 cites W1988824186 @default.
• W2020350080 cites W1993232032 @default.
• W2020350080 cites W1993580604 @default.
• W2020350080 cites W1995507236 @default.
• W2020350080 cites W2006239481 @default.
• W2020350080 cites W2007509302 @default.
• W2020350080 cites W2008029604 @default.
• W2020350080 cites W2009888336 @default.
• W2020350080 cites W2037227062 @default.
• W2020350080 cites W2060671095 @default.
• W2020350080 cites W2061600983 @default.
• W2020350080 cites W2062268212 @default.
• W2020350080 cites W2066790649 @default.
• W2020350080 cites W2070824171 @default.
• W2020350080 cites W2071844171 @default.
• W2020350080 cites W2072400772 @default.
• W2020350080 cites W2082471981 @default.
• W2020350080 cites W2090904641 @default.
• W2020350080 cites W2100269253 @default.
• W2020350080 cites W2101072720 @default.
• W2020350080 cites W2102298863 @default.
• W2020350080 cites W2102633875 @default.
• W2020350080 cites W2105631023 @default.
• W2020350080 cites W2105746559 @default.
• W2020350080 cites W2110937119 @default.
• W2020350080 cites W2112666597 @default.
• W2020350080 cites W2113638068 @default.
• W2020350080 cites W2118650242 @default.
• W2020350080 cites W2121014368 @default.
• W2020350080 cites W2123979501 @default.
• W2020350080 cites W2124803006 @default.
• W2020350080 cites W2130395505 @default.
• W2020350080 cites W2134268527 @default.
• W2020350080 cites W2135409720 @default.
• W2020350080 cites W2137587326 @default.
• W2020350080 cites W2138840232 @default.
• W2020350080 cites W2139602514 @default.
• W2020350080 cites W2148479501 @default.
• W2020350080 cites W2149220222 @default.
• W2020350080 cites W2154894697 @default.
• W2020350080 cites W2163800490 @default.
• W2020350080 cites W2168675699 @default.
• W2020350080 cites W2171059311 @default.
• W2020350080 doi "https://doi.org/10.1371/journal.pone.0043584" @default.
• W2020350080 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3422254" @default.
• W2020350080 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22912891" @default.
• W2020350080 hasPublicationYear "2012" @default.
• W2020350080 type Work @default.
• W2020350080 sameAs 2020350080 @default.
• W2020350080 citedByCount "34" @default.
• W2020350080 countsByYear W20203500802013 @default.
• W2020350080 countsByYear W20203500802014 @default.
• W2020350080 countsByYear W20203500802015 @default.
• W2020350080 countsByYear W20203500802016 @default.
• W2020350080 countsByYear W20203500802017 @default.
• W2020350080 countsByYear W20203500802018 @default.
• W2020350080 countsByYear W20203500802019 @default.
• W2020350080 countsByYear W20203500802020 @default.
• W2020350080 countsByYear W20203500802021 @default.
• W2020350080 countsByYear W20203500802022 @default.
• W2020350080 countsByYear W20203500802023 @default.
• W2020350080 crossrefType "journal-article" @default.
• W2020350080 hasAuthorship W2020350080A5006529578 @default.
• W2020350080 hasAuthorship W2020350080A5025632358 @default.
• W2020350080 hasAuthorship W2020350080A5042634912 @default.
• W2020350080 hasAuthorship W2020350080A5045660349 @default.
• W2020350080 hasAuthorship W2020350080A5063407939 @default.
• W2020350080 hasAuthorship W2020350080A5073558224 @default.
• W2020350080 hasBestOaLocation W20203500801 @default.
• W2020350080 hasConcept C104317684 @default.
• W2020350080 hasConcept C127561419 @default.
• W2020350080 hasConcept C133717845 @default.
• W2020350080 hasConcept C1491633281 @default.

• next