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- W2020351307 endingPage "B162" @default.
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- W2020351307 abstract "Simple and label-free electrochemical sensors for the detection of DNA hybridization were developed based on charge perturbation of poly(thionine)-modified glassy carbon and gold electrodes (PTH/GCE and PTH/Au). Probe DNA (pDNA) was immobilized on PTH films via covalent bonds between the amine (-NH2) group of the PTH and the phosphate (PO43−) group at the 5' end of the pDNA. The immobilized single-strand pDNA decreased the oxidation current of [Fe(CN)6]4− as a result of electrostatic repulsion by the negatively charged PO43- backbone of the pDNA. The current was further decreased subsequent to duplex formation by the hybridization of target complementary DNA (cDNA) due to increased electrostatic repulsion. Both sensors demonstrated excellent selectivity, stability, and the sensitivity of 1.27 and 58.6 μA·cm−2·pM−1 with detection limits of 0.47 and 0.20 pM, respectively, for cDNA hybridization." @default.
- W2020351307 created "2016-06-24" @default.
- W2020351307 creator A5040199135 @default.
- W2020351307 creator A5061529534 @default.
- W2020351307 creator A5081650165 @default.
- W2020351307 date "2015-01-01" @default.
- W2020351307 modified "2023-10-17" @default.
- W2020351307 title "Label-Free Detection of DNA Hybridization by Using Charge Perturbation on Poly(thionine)-Modified Glassy Carbon and Gold Electrodes" @default.
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- W2020351307 doi "https://doi.org/10.1149/2.0291507jes" @default.
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