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- W2020356246 abstract "An enzyme-linked immunosorbent assay (ELISA) for the detection of diacetoxyscirpenol (DAS) was developed. Antibodies against DAS were obtained after immunization of rabbits with DAS-hemiglutarate-human serum albumin (DAS-HG-HSA). After an immunization period of 6 weeks a sufficient level of specific antibodies was reached. A DAS-hemisuccinate-horseradish peroxidase-conjugate (DAS-HS-HRP) was prepared by an ester method and used as enzyme-labelled toxin in the competetive assay The detection limit for DAS was about 10 pg/ml. The relative cross reactivity of the assay was 597.5, 5.2, 100.0, 2.5, and 1.5% for 3α-acetyl-DAS, DAS, T-2 toxin, neosolaniol, and 15-acetoxyscirpenol, respectively." @default.
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- W2020356246 date "1988-02-01" @default.
- W2020356246 modified "2023-10-09" @default.
- W2020356246 title "Development of a sensitive enzyme-linked immunosorbent assay for the detection of diacetoxyscirpenol" @default.
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- W2020356246 doi "https://doi.org/10.1016/0168-1605(88)90079-7" @default.
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