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• W2020391719 endingPage "152" @default.
• W2020391719 startingPage "143" @default.
• W2020391719 abstract "Premutational lesions produced by ultraviolet radiation in the Gln2 tRNA genes of E. coli B/r show differing sensitivities to a mutation avoidance phenomenon known as mutation frequency decline (MFD). A mutation event that changes the wild-type gene to an amber (UAG) suppressor is normally sensitive to MFD. Mutation of this amber suppressor to an ochre (UAA) suppressor is not sensitive to MFD. These two mutation events occur in the same anticodon region of the DNA. The dissimilarity of MFD sensitivity between these two mutations may result because of the respective premutational photoproducts for the two are located in opposite strands of duplex DNA. To examine the effect of strand position of the premutational lesions of MFD, recombinant lambda phage were constructed that contained the amber suppressor as a mutation target in the two possible orientations. Comparison of MFD in bacterial lysogens containing either of the two types of recombinant prophage indicated that reversing the orientation of the target sequence relative to adjacent bacterial DNA had no effect on MFD. Since rotational inversion of the target sequence did not alter the sensitivity to MFD of mutation occurring at the cloned target gene, the antimutation process inherent to FMD can not be attributed to an asymmetrical interaction between the template strands and the DNA-replication complex." @default.
• W2020391719 created "2016-06-24" @default.
• W2020391719 creator A5007319030 @default.
• W2020391719 creator A5010315369 @default.
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• W2020391719 date "1984-11-01" @default.
• W2020391719 modified "2023-09-26" @default.
• W2020391719 title "UV-Mutagenesis at a cloned target sequence: Converted suppressor mutation is insensitive to mutation frequency decline regardless of the gene orientation" @default.
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• W2020391719 doi "https://doi.org/10.1016/0167-8817(84)90032-4" @default.
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