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- W2020397992 abstract "Phalloidin labelled with trimethyl rhodamine isothiocyanate in extraction buffer was used to stain actin in suspension cells of tobacco. Confocal immunofluorescence microscopy indicated the presence of filamentous actin containing structures in interphase nuclei of elongating cells of Nicotiana tabacum ‘Bright Yellow 2 Go’. The results were not affected by the omission of DMSO from the extraction medium. These structures, called actin baskets, were present in about 20% of the cells after induced elongation and varied in size, shape, and number per nucleus. The cytoplasmic actin array remained intact. It is proposed that the baskets have a transient character and are related to differentiation. Key words: confocal laser scanning microscopy, elongation, F-actin, nucleus, protoplasts, TRITC–phalloidin." @default.
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- W2020397992 title "Basket-shaped structures formed by F-actin in the nuclei of elongating cells of Nicotiana tabacum" @default.
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- W2020397992 doi "https://doi.org/10.1139/b93-084" @default.
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