FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2020450167> ?p ?o ?g. }

• W2020450167 endingPage "1402" @default.
• W2020450167 startingPage "1395" @default.
• W2020450167 abstract "In mammals, Sry is the master regulator of male sex determination, although how it functions is still unclear. By contrast, female sex determination depends on the action of Rspo1 and Wnt4, the regulators of Wnt/β-catenin signaling. To seek a possible interaction between male and female sex determination mechanisms, we examined whether Sry affects Wnt/β-catenin signaling. Using the TOPFLASH reporter system to measure Lef/Tcf-dependent transcriptional activity, we showed that ectopic expression of mouse Sry strongly suppressed Wnt/β-catenin signaling in mouse embryonal carcinoma and human embryonic kidney cell lines. This inhibition occurred downstream of β-catenin but upstream of Lef/Tcf, and depended on both the HMG-box and the C-terminal transcriptional activation domain. By contrast, TOPFLASH was not inhibited by human SRY, which apparently lacks a transcriptional activation domain. However, a fusion construct consisting of human SRY attached to the C-terminal domain of mouse Sry was able to inhibit TOPFLASH effectively. Furthermore, Sry constructs carrying point mutations equivalent to those in human sex reversal mutations were less effective in inhibiting Wnt/β-catenin signaling. Also, we showed that the action of Sry as a transcriptional activator was both necessary and sufficient to inhibit Wnt/β-catenin signaling, suggesting that the transcriptional targets of Sry are responsible for the inhibition of signaling. Sox9 is a potential transcriptional target of Sry, although quantitative RT-PCR analysis indicates that the expression of Sox9 was not up-regulated by the ectopic expression of mouse Sry in mouse embryonal carcinoma cells. While the present study demonstrates an impact of mouse Sry on Wnt/β-catenin signaling at an in vitro level, it requires further investigations to assess whether such action also takes place in vivo to regulate male sex determination." @default.
• W2020450167 created "2016-06-24" @default.
• W2020450167 creator A5021297657 @default.
• W2020450167 creator A5072356526 @default.
• W2020450167 creator A5088570602 @default.
• W2020450167 date "2008-12-01" @default.
• W2020450167 modified "2023-09-27" @default.
• W2020450167 title "Ectopic expression of mouse Sry interferes with Wnt/β-catenin signaling in mouse embryonal carcinoma cell lines" @default.
• W2020450167 cites W1498970130 @default.
• W2020450167 cites W1538623601 @default.
• W2020450167 cites W1584850257 @default.
• W2020450167 cites W1600280267 @default.
• W2020450167 cites W1635607892 @default.
• W2020450167 cites W1867052108 @default.
• W2020450167 cites W1965719589 @default.
• W2020450167 cites W1973295687 @default.
• W2020450167 cites W1973422437 @default.
• W2020450167 cites W1975716064 @default.
• W2020450167 cites W1983300728 @default.
• W2020450167 cites W1983712244 @default.
• W2020450167 cites W1984220883 @default.
• W2020450167 cites W1987046160 @default.
• W2020450167 cites W2002812966 @default.
• W2020450167 cites W2007969716 @default.
• W2020450167 cites W2008801609 @default.
• W2020450167 cites W2008812172 @default.
• W2020450167 cites W2010217881 @default.
• W2020450167 cites W2012039807 @default.
• W2020450167 cites W2023002592 @default.
• W2020450167 cites W2026598920 @default.
• W2020450167 cites W2034010221 @default.
• W2020450167 cites W2039248606 @default.
• W2020450167 cites W2049424035 @default.
• W2020450167 cites W2050031444 @default.
• W2020450167 cites W2051669225 @default.
• W2020450167 cites W2053444110 @default.
• W2020450167 cites W2062932581 @default.
• W2020450167 cites W2068098232 @default.
• W2020450167 cites W2090636859 @default.
• W2020450167 cites W2095209530 @default.
• W2020450167 cites W2100070586 @default.
• W2020450167 cites W2100192772 @default.
• W2020450167 cites W2101027724 @default.
• W2020450167 cites W2101332045 @default.
• W2020450167 cites W2102814715 @default.
• W2020450167 cites W2102828359 @default.
• W2020450167 cites W2110510042 @default.
• W2020450167 cites W2118249097 @default.
• W2020450167 cites W2123037811 @default.
• W2020450167 cites W2124943870 @default.
• W2020450167 cites W2125639392 @default.
• W2020450167 cites W2141175150 @default.
• W2020450167 cites W2146167376 @default.
• W2020450167 cites W2152501601 @default.
• W2020450167 cites W2156726032 @default.
• W2020450167 cites W2159231404 @default.
• W2020450167 cites W2163398664 @default.
• W2020450167 cites W2222903824 @default.
• W2020450167 doi "https://doi.org/10.1016/j.bbagen.2008.07.001" @default.
• W2020450167 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2637177" @default.
• W2020450167 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18675318" @default.
• W2020450167 hasPublicationYear "2008" @default.
• W2020450167 type Work @default.
• W2020450167 sameAs 2020450167 @default.
• W2020450167 citedByCount "21" @default.
• W2020450167 countsByYear W20204501672012 @default.
• W2020450167 countsByYear W20204501672013 @default.
• W2020450167 countsByYear W20204501672015 @default.
• W2020450167 countsByYear W20204501672017 @default.
• W2020450167 countsByYear W20204501672018 @default.
• W2020450167 countsByYear W20204501672019 @default.
• W2020450167 crossrefType "journal-article" @default.
• W2020450167 hasAuthorship W2020450167A5021297657 @default.
• W2020450167 hasAuthorship W2020450167A5072356526 @default.
• W2020450167 hasAuthorship W2020450167A5088570602 @default.
• W2020450167 hasBestOaLocation W20204501672 @default.
• W2020450167 hasConcept C104317684 @default.
• W2020450167 hasConcept C137620995 @default.
• W2020450167 hasConcept C153911025 @default.
• W2020450167 hasConcept C2778486617 @default.
• W2020450167 hasConcept C2780246931 @default.
• W2020450167 hasConcept C2781430442 @default.
• W2020450167 hasConcept C502942594 @default.
• W2020450167 hasConcept C54355233 @default.
• W2020450167 hasConcept C62478195 @default.
• W2020450167 hasConcept C73456138 @default.
• W2020450167 hasConcept C86339819 @default.
• W2020450167 hasConcept C86803240 @default.
• W2020450167 hasConcept C95444343 @default.
• W2020450167 hasConcept C97037327 @default.
• W2020450167 hasConceptScore W2020450167C104317684 @default.
• W2020450167 hasConceptScore W2020450167C137620995 @default.
• W2020450167 hasConceptScore W2020450167C153911025 @default.
• W2020450167 hasConceptScore W2020450167C2778486617 @default.
• W2020450167 hasConceptScore W2020450167C2780246931 @default.
• W2020450167 hasConceptScore W2020450167C2781430442 @default.
• W2020450167 hasConceptScore W2020450167C502942594 @default.
• W2020450167 hasConceptScore W2020450167C54355233 @default.

• next