Matches in SemOpenAlex for { <https://semopenalex.org/work/W2020450503> ?p ?o ?g. }
- W2020450503 endingPage "126" @default.
- W2020450503 startingPage "118" @default.
- W2020450503 abstract "Fibroblast migration into the blood clot initially filling a wound requires close interaction between fibroblasts and the matrix of the fibrin clot. However, very little is known about the specific receptor–ligand interactions that mediate fibroblast attachment to fibrin. Using an attachment assay developed to measure even relatively weak interactions, we demonstrate here that normal human dermal fibroblasts can attach to substrates coated with fibrinogen, fibrin, or the fibrinogen breakdown product I-9D. Fibroblast attachment to these ligands did not require the presence of fibronectin on the cell surface or as a component of the substrate. Cells treated with cycloheximide and monensin, to limit the synthesis and secretion of endogenous fibronectin, attached as well as untreated cells. The synthetic peptide GRGDS inhibited adhesion to fibrinogen, fibrin, and fibrinogen I-9D by about 60%, while the control peptide GRGES had no substantial effect. We conclude that attachment to these ligands is mediated at least partially by direct interactions between the substrates and one specific receptor, the integrin αvβ3. Affinity chromatography demonstrated that αvβ3 from detergent lysates of fibroblasts bound to a fibrinogen matrix and was eluted with EDTA. Furthermore, antibodies against the αvβ3 complex or against the αv subunit inhibited fibroblast attachment to fibrinogen and fibrin by 50–70%. An inhibitory antibody against the integrin β1 subunit had no effect. The observation that integrin antagonists could not produce complete inhibition suggests that there may be other fibroblast cell surface proteins that can bind directly to fibrinogen." @default.
- W2020450503 created "2016-06-24" @default.
- W2020450503 creator A5021540608 @default.
- W2020450503 creator A5026928060 @default.
- W2020450503 creator A5073314507 @default.
- W2020450503 creator A5074426732 @default.
- W2020450503 creator A5076772705 @default.
- W2020450503 creator A5080650253 @default.
- W2020450503 date "1997-04-01" @default.
- W2020450503 modified "2023-10-14" @default.
- W2020450503 title "Human Fibroblasts Bind Directly to Fibrinogen at RGD Sites through Integrin αvβ3" @default.
- W2020450503 cites W1483915559 @default.
- W2020450503 cites W1568828918 @default.
- W2020450503 cites W1571617379 @default.
- W2020450503 cites W1577857859 @default.
- W2020450503 cites W1588491159 @default.
- W2020450503 cites W1598287593 @default.
- W2020450503 cites W1604526865 @default.
- W2020450503 cites W1649140242 @default.
- W2020450503 cites W1650068461 @default.
- W2020450503 cites W166803931 @default.
- W2020450503 cites W1965888938 @default.
- W2020450503 cites W1969540615 @default.
- W2020450503 cites W1969632860 @default.
- W2020450503 cites W1973834594 @default.
- W2020450503 cites W1981284178 @default.
- W2020450503 cites W1995282093 @default.
- W2020450503 cites W2003997440 @default.
- W2020450503 cites W2006878196 @default.
- W2020450503 cites W2017734122 @default.
- W2020450503 cites W2024373128 @default.
- W2020450503 cites W2029231027 @default.
- W2020450503 cites W2037986253 @default.
- W2020450503 cites W2039012502 @default.
- W2020450503 cites W2044806537 @default.
- W2020450503 cites W2044977073 @default.
- W2020450503 cites W2047196924 @default.
- W2020450503 cites W2066367871 @default.
- W2020450503 cites W2067694623 @default.
- W2020450503 cites W2087342203 @default.
- W2020450503 cites W2090637083 @default.
- W2020450503 cites W2109505295 @default.
- W2020450503 cites W2114246972 @default.
- W2020450503 cites W2125258660 @default.
- W2020450503 cites W2159822998 @default.
- W2020450503 cites W2169170805 @default.
- W2020450503 cites W2287104688 @default.
- W2020450503 cites W4245860073 @default.
- W2020450503 doi "https://doi.org/10.1006/excr.1997.3512" @default.
- W2020450503 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9141628" @default.
- W2020450503 hasPublicationYear "1997" @default.
- W2020450503 type Work @default.
- W2020450503 sameAs 2020450503 @default.
- W2020450503 citedByCount "137" @default.
- W2020450503 countsByYear W20204505032012 @default.
- W2020450503 countsByYear W20204505032013 @default.
- W2020450503 countsByYear W20204505032014 @default.
- W2020450503 countsByYear W20204505032015 @default.
- W2020450503 countsByYear W20204505032016 @default.
- W2020450503 countsByYear W20204505032017 @default.
- W2020450503 countsByYear W20204505032018 @default.
- W2020450503 countsByYear W20204505032019 @default.
- W2020450503 countsByYear W20204505032020 @default.
- W2020450503 countsByYear W20204505032021 @default.
- W2020450503 countsByYear W20204505032022 @default.
- W2020450503 countsByYear W20204505032023 @default.
- W2020450503 crossrefType "journal-article" @default.
- W2020450503 hasAuthorship W2020450503A5021540608 @default.
- W2020450503 hasAuthorship W2020450503A5026928060 @default.
- W2020450503 hasAuthorship W2020450503A5073314507 @default.
- W2020450503 hasAuthorship W2020450503A5074426732 @default.
- W2020450503 hasAuthorship W2020450503A5076772705 @default.
- W2020450503 hasAuthorship W2020450503A5080650253 @default.
- W2020450503 hasConcept C153911025 @default.
- W2020450503 hasConcept C170493617 @default.
- W2020450503 hasConcept C189165786 @default.
- W2020450503 hasConcept C195687474 @default.
- W2020450503 hasConcept C202751555 @default.
- W2020450503 hasConcept C203014093 @default.
- W2020450503 hasConcept C2777292125 @default.
- W2020450503 hasConcept C2778005187 @default.
- W2020450503 hasConcept C2778128677 @default.
- W2020450503 hasConcept C2779036427 @default.
- W2020450503 hasConcept C2780381497 @default.
- W2020450503 hasConcept C3675279 @default.
- W2020450503 hasConcept C54173615 @default.
- W2020450503 hasConcept C55493867 @default.
- W2020450503 hasConcept C86492073 @default.
- W2020450503 hasConcept C86803240 @default.
- W2020450503 hasConcept C89560881 @default.
- W2020450503 hasConcept C95444343 @default.
- W2020450503 hasConceptScore W2020450503C153911025 @default.
- W2020450503 hasConceptScore W2020450503C170493617 @default.
- W2020450503 hasConceptScore W2020450503C189165786 @default.
- W2020450503 hasConceptScore W2020450503C195687474 @default.
- W2020450503 hasConceptScore W2020450503C202751555 @default.
- W2020450503 hasConceptScore W2020450503C203014093 @default.
- W2020450503 hasConceptScore W2020450503C2777292125 @default.