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- W2020470451 abstract "The collective behavior of bacteria plays an important role in the biofilm development. It is essential to investigate the dynamics of bacterial colonization and the interactions between the bacteria and the physical parameters of the substrate and fluidic biosurfactants as well. Recently, studies on prokaryotic cell biology have been facilitated with the advancement of quantitative microscopic imaging techniques. In this study, the fluidic properties of the biofilms formed in Escherichia coli (E.coli) and Bacillus subtilis (B.subtilis) colonies were compared by visualizing 200-nm fluorescent beads which were initially embedded in the agar plate and distributed spontaneously on the upper surface of the growing colonies. We focus on finding the effect of the substrate stiffness on their swarming motility and on the hydrodynamics of the fluidic biofilm by long-term live cell imaging of B.subtilis cells grown on an agar gel substrate. The viable cell number and motility of the bacteria in the colony were measured by phase contrast imaging and multiple particle tracking techniques. It was found that the bacteria in the biofilm have a property to switch between the growth and swarming phase. Vortical flow patterns near the edge of the colony were observed clearly by tracking of the beads moving in the biofilm of the B.subtilis colony, whereas there was no movement of the beads in the E.coli colony. Further analysis with a cross-correlation method revealed the detailed velocity vector field of the thin biofilm surrounding the B.subtilis cells. The present study should be a first step to find the effect of the fluidic biofilm on the growth and the swarming dynamics of bacteria, and to uncover a novel physical factor that regulates bacterial cell physiology." @default.
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- W2020470451 date "2012-01-01" @default.
- W2020470451 modified "2023-09-27" @default.
- W2020470451 title "Visualization of Vortical Flow Patterns in a Thin Fluidic Biofilm Surrounding a Bacillus Subtilis Bacterial Colony" @default.
- W2020470451 doi "https://doi.org/10.1016/j.bpj.2011.11.830" @default.
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