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- W2020480028 abstract "The use of reverse transcriptase in conjunction with the polymerase chain reaction (RT-PCR) has proven invaluable in the analysis of the T cell receptor (TCR) repertoire of different populations of T cells. However, the presence of a variable region in the T cell receptor has hindered the design of primers for the 5′ end of the TCR cDNA. We describe the design and use of a degenerate consensus primer that allows amplification of both the α and β chains of the human TCR. We have used this primer in the analysis of the TCR distribution of T cell clones, peripheral blood lymphocytes and lymphocytes residing in tissue. In addition, the primer has allowed the identification of an alternative splice site in the β chain constant region which cannot translate into a functional constant region. We have found the primer to be easy to use, sensitive and specific." @default.
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- W2020480028 modified "2023-09-23" @default.
- W2020480028 title "A consensus primer to amplify both α and β chains of the human T cell receptor" @default.
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- W2020480028 doi "https://doi.org/10.1016/0022-1759(94)90123-6" @default.
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