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• W2020484895 abstract "A novel method of estimating the kinetic parameters of Taq DNA polymerase during rapid cycle PCR is presented. A model was constructed using a simplified sigmoid function to represent substrate accumulation during PCR in combination with the general equation describing high substrate inhibition for Michaelis-Menten enzymes. The PCR progress curve was viewed as a series of independent reactions where initial rates were accurately measured for each cycle. Kinetic parameters were obtained for allele-specific PCR (AS-PCR) amplification to examine the effect of mismatches on amplification. A high degree of correlation was obtained providing evidence of substrate inhibition as a major cause of the plateau phase that occurs in the later cycles of PCR." @default.
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• W2020484895 date "2002-12-01" @default.
• W2020484895 modified "2023-09-25" @default.
• W2020484895 title "Kinetic characterisation of primer mismatches in allele-specific PCR: a quantitative assessment" @default.
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• W2020484895 doi "https://doi.org/10.1016/s0006-291x(02)02750-x" @default.
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