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- W2020489301 abstract "Calcineurin was dissociated into subunits A and B by SDS and the dissociated subunits were separated by Sephadex G‐100 column chromatography in SDS. The phosphatase activity was associated with the A subunit and was detected only in the presence of MnCl 2 of the various divalent cations tested. The Mn 2+ ‐dependent phosphatase of A subunit was stimulated (4‐5‐fold) by calmodulin. The subunit B increased only modestly Mn 2+ stimulated phosphatase activity of subunit A but markedly increased it when assay also contained calmodulin. These results support the view that subunit B plays an important role in Mn 2+ /calmodulin regulation of subunit A phosphatase activity. They also lend further support to our earlier postulate ([1984] FEBS Lett. 169, 251‐255) that Mn 2+ is a powerful regulator of calcineurin phosphatase." @default.
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- W2020489301 title "Resolution of bovine brain calcineurin subunits: stimulatory effect of subunit B on subunit A phosphatase activity" @default.
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