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• W2020490094 abstract "Using a series of position and nucleotide variants of the SV40 late polyadenylation signal we have demonstrated that three sequence elements determine the precise site of 3′-end cleavage in mammalian premRNAs: an upstream AAUAAA element, a downstream U-rich element consisting of five nucleotides, at least four of which are uridine, and a nucleotide preference at the site of cleavage in the order A>U> C>> G. Cleavage occurs no closer than 11 bases, but no farther than 23 bases from the AAUAAA element. The downstream U-rich element is usually located 10–30 bases from the cleavage site. The relative position of the AAUAAA and the U-rich elements define the approximate region within a 13 base domain in which cleavage will occur. The exact position of cleavage Is then determined by the local nucleotide sequence In the order of preference noted above. This model accounts for nearly three quarters of olyadenylation signals surveyed and is consistent with previous experimental observations." @default.
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• W2020490094 date "1995-01-01" @default.
• W2020490094 modified "2023-10-13" @default.
• W2020490094 title "Cleavage site determinants min the mammalian polydenylation signal" @default.
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• W2020490094 doi "https://doi.org/10.1093/nar/23.14.2614" @default.
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