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• W2020502347 abstract "Abstract: Several recent theories and simulations have predicted that shear flow could enhance, or, conversely, suppress the nucleation of crystals from solution. Such modulations would offer a pathway for nucleation control and provide a novel explanation for numerous mysteries in nucleation research. For experimental tests of the effects of shear flow on protein crystal nucleation, we found that if a protein solution droplet of ∼ 5 μL (2–3 mm diameter at base) is held on a hydrophobic substrate in an enclosed environment and in a quasi-uniform constant electric field of 2 to 6 kV cm−1, a rotational flow with a maximum rate at the droplet top of ∼ 10 μm s−1 is induced. The shear rate varies from 10−3 to 10−1 s−1. The likely mechanism of the rotational flow involves adsorption of the protein and amphiphylic buffer molecules on the air–water interface and their redistribution in the electric field, leading to nonuniform surface tension of the droplet and surface tension-driven flow. Observations of the number of nucleated crystals in 24- and 72-h experiments with the proteins ferritin, apoferritin, and lysozyme revealed that the crystals are typically nucleated at a certain radius of the droplet, that is, at a preferred shear rate. Variations of the rotational flow velocity resulted in suppression or enhancement of the total number of nucleated crystals of ferritin and apoferritin, while all solution flow rates were found to enhance lysozyme crystal nucleation. These observations show that shear flow may strongly affect nucleation, and that for some systems, an optimal flow velocity, leading to fastest nucleation, exists. Comparison with the predictions of theories and simulations suggest that the formation of ordered nuclei in a “normal” protein solution cannot be affected by such low shear rates. We conclude that the flow acts by helping or suppressing the formation of ordered nuclei within mesoscopic metastable dense liquid clusters. Such clusters were recently shown to exist in protein solutions and to constitute the first step in the nucleation mechanism of many protein and nonprotein systems." @default.
• W2020502347 created "2016-06-24" @default.
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• W2020502347 date "2006-09-01" @default.
• W2020502347 modified "2023-09-26" @default.
• W2020502347 title "Nucleation of Protein Crystals under the Influence of Solution Shear Flow" @default.
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• W2020502347 doi "https://doi.org/10.1196/annals.1362.048" @default.
• W2020502347 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17124126" @default.
• W2020502347 hasPublicationYear "2006" @default.
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