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- W2020504414 abstract "Human and monkey corneal epithelia were investigated for lipoxygenases. The low-speed supernatant of human corneal epithelium converted arachidonic acid to one prominent metabolite, which was identified as 15-hydroxyeicosatetraenoic acid by gas chromarography mass spectrometry and by ultra violet analyses. Eicosatetraynoic acid, a lipoxygenase inhibitor, reduced the formation of 15-hydroxyeicosatetraenoic acid, by 90% at 30 μM, while esculetin (30 μM) and diclofenac (100 μM) did not inhibit the biosynthesis. Immunohistochemical analysis of human cornea using a polyclonal antibody against recombinant human reticulocyte 15-lipoxygenase showed staining of the epithelium, but not the stroma. The cytosolic fraction of epithelial cells of the monkey, Macaca fascicularis, converted [1-14C]arachidonic acid to two major metabolites, which were identified as 15S-hydroxyeicosatetraenoic and 12S-hydroxyeicosatetraenoic acids by cochromatography with these compounds on reverse-phase, straight-phase and chiral high-performance liquid chromatography. Lipoxygenase activity could not be detected in the microsomal fraction. The results provide biochemical, pharmacological and immunohistological evidence of a 15-lipoxygenase in human corneal epithelium and suggest that monkey corneal epithelium contains cytosolic 12- and 15-lipoxygenases." @default.
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- W2020504414 date "1994-09-01" @default.
- W2020504414 modified "2023-09-29" @default.
- W2020504414 title "Lipoxygenases in Corneal Epithelia of Man and Cynomolgus Monkey" @default.
- W2020504414 doi "https://doi.org/10.1006/exer.1994.1113" @default.
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