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• W2020506568 endingPage "490" @default.
• W2020506568 startingPage "476" @default.
• W2020506568 abstract "Many green fluorescent protein (GFP) variants have been developed for use as fluorescent tags, and recently a superfolder GFP (sfGFP) has been developed as a robust folding reporter. This new variant shows increased stability and improved folding kinetics, as well as 100% recovery of native protein after denaturation. Here, we characterize sfGFP, and find that this variant exhibits hysteresis as unfolding and refolding equilibrium titration curves are non-coincident even after equilibration for more than eight half-lives as estimated from kinetic unfolding and refolding studies. This hysteresis is attributed to trapping in a native-like intermediate state. Mutational studies directed towards inhibiting chromophore formation indicate that the novel backbone cyclization is responsible for the hysteresis observed in equilibrium titrations of sfGFP. Slow equilibration and the presence of intermediates imply a rough landscape. However, de novo folding in the absence of the chromophore is dominated by a smoother energy landscape than that sampled during unfolding and refolding of the post-translationally modified polypeptide." @default.
• W2020506568 created "2016-06-24" @default.
• W2020506568 creator A5006148975 @default.
• W2020506568 creator A5043151535 @default.
• W2020506568 creator A5044823225 @default.
• W2020506568 creator A5053680151 @default.
• W2020506568 creator A5068448796 @default.
• W2020506568 date "2007-10-01" @default.
• W2020506568 modified "2023-10-17" @default.
• W2020506568 title "The Rough Energy Landscape of Superfolder GFP Is Linked to the Chromophore" @default.
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• W2020506568 cites W1482642935 @default.
• W2020506568 cites W1519297291 @default.
• W2020506568 cites W1522770875 @default.
• W2020506568 cites W1968218728 @default.
• W2020506568 cites W1969262554 @default.
• W2020506568 cites W1969554454 @default.
• W2020506568 cites W1971481651 @default.
• W2020506568 cites W1971966571 @default.
• W2020506568 cites W1972459558 @default.
• W2020506568 cites W1972675000 @default.
• W2020506568 cites W1974144765 @default.
• W2020506568 cites W1983046430 @default.
• W2020506568 cites W1985118646 @default.
• W2020506568 cites W1986593253 @default.
• W2020506568 cites W1986927536 @default.
• W2020506568 cites W2000124531 @default.
• W2020506568 cites W2000430596 @default.
• W2020506568 cites W2001741929 @default.
• W2020506568 cites W2001812046 @default.
• W2020506568 cites W2003813143 @default.
• W2020506568 cites W2004373243 @default.
• W2020506568 cites W2005717963 @default.
• W2020506568 cites W2006860644 @default.
• W2020506568 cites W2010637993 @default.
• W2020506568 cites W2011673070 @default.
• W2020506568 cites W2017639307 @default.
• W2020506568 cites W2019248292 @default.
• W2020506568 cites W2028014512 @default.
• W2020506568 cites W2029377901 @default.
• W2020506568 cites W2032579724 @default.
• W2020506568 cites W2033458944 @default.
• W2020506568 cites W2035879549 @default.
• W2020506568 cites W2036977073 @default.
• W2020506568 cites W2038540528 @default.
• W2020506568 cites W2040022655 @default.
• W2020506568 cites W2040362675 @default.
• W2020506568 cites W2041017870 @default.
• W2020506568 cites W2041106046 @default.
• W2020506568 cites W2041914510 @default.
• W2020506568 cites W2043925446 @default.
• W2020506568 cites W2046408940 @default.
• W2020506568 cites W2050461122 @default.
• W2020506568 cites W2059444968 @default.
• W2020506568 cites W2060103222 @default.
• W2020506568 cites W2061104342 @default.
• W2020506568 cites W2062714819 @default.
• W2020506568 cites W2063616898 @default.
• W2020506568 cites W2064446384 @default.
• W2020506568 cites W2070929467 @default.
• W2020506568 cites W2073055599 @default.
• W2020506568 cites W2073808757 @default.
• W2020506568 cites W2078632235 @default.
• W2020506568 cites W2079630207 @default.
• W2020506568 cites W2081344793 @default.
• W2020506568 cites W2085756960 @default.
• W2020506568 cites W2087823077 @default.
• W2020506568 cites W2091385808 @default.
• W2020506568 cites W2091634218 @default.
• W2020506568 cites W2092318974 @default.
• W2020506568 cites W2096691349 @default.
• W2020506568 cites W2102074396 @default.
• W2020506568 cites W2106518073 @default.
• W2020506568 cites W2106762118 @default.
• W2020506568 cites W2115803723 @default.
• W2020506568 cites W2123440527 @default.
• W2020506568 cites W2127068865 @default.
• W2020506568 cites W2140213897 @default.
• W2020506568 cites W2141726589 @default.
• W2020506568 cites W2149744037 @default.
• W2020506568 cites W2151796887 @default.
• W2020506568 cites W2152833660 @default.
• W2020506568 cites W2158476854 @default.
• W2020506568 cites W2160772046 @default.
• W2020506568 cites W2161996750 @default.
• W2020506568 cites W2169515696 @default.
• W2020506568 cites W2171610577 @default.
• W2020506568 cites W2171755502 @default.
• W2020506568 cites W2171999720 @default.
• W2020506568 cites W4233808990 @default.
• W2020506568 cites W4239652312 @default.
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• W2020506568 doi "https://doi.org/10.1016/j.jmb.2007.07.071" @default.
• W2020506568 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2695656" @default.
• W2020506568 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17822714" @default.
• W2020506568 hasPublicationYear "2007" @default.
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