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• W2020546800 abstract "Nogami S., Hayashi Y., Korenaga M., Tada I. and tanaka H. 1988. Monoclonal antibodies specific for Onchocerca volvulus as determined by immunofluorescence. International Journal for Parasitology18: 503–507. A total of four murine monoclonal antibodies (mAb) was determined to be specific for Onchocerca volvulus when examined by immunofluorescence. Adult Ascaris suum as antigen was used to eliminate cross-reactive mAb during screening of clonal products from mice immunized with O. volvulus extracts. The selected mAbs were further examined for specificity using 13 species of filarial and non-filarial helminths. Four mAbs, non-reactive to A. suum, reacted to O. volvulus alone but did not react to the other 12 helminths, suggesting that A. suum may be an effective tool for selecting O. volvulus-specific mAb. The isotypes of these four mAbs were all IgG; i.e. three were of the IgG1 subclass and one was IgG2a. Worm antigen sites bound by these specific mAbs were all in the intestinal tissue of adult O. volvulus. Analysis with polyacrylamide slab gel electrophoresis in sodium dodecylsulfate (SDS-PAGE) and immunoblotting revealed that an epitope specific for O. volvulus exists on a Mr 27,000 polypeptide." @default.
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• W2020546800 date "1988-06-01" @default.
• W2020546800 modified "2023-10-03" @default.
• W2020546800 title "Monoclonal antibodies specific for Onchocerca volvulus as determined by immunofluorescence" @default.
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• W2020546800 doi "https://doi.org/10.1016/0020-7519(88)90014-8" @default.
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