Matches in SemOpenAlex for { <https://semopenalex.org/work/W2020563079> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W2020563079 abstract "Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DCThe majority of breast cancers diagnosed in the United States are estrogen receptor positive (ER+). These patients may respond to targeted endocrine therapies, but up to 30% of patients experience a cancer recurrence. We hypothesized that the molecular interaction of ER+ breast cancer cells with mammary fibroblasts might lead to endocrine resistance and, eventually, breast cancer relapse. We developed a three-dimensional (3D) co-culture model representing cancer cell-fibroblast interactions during early tumor invasion. We show that the proliferation of MCF7 ER+ cells switches from hormone-dependent to growth factor-dependent in the presence of human mammary fibroblasts. Further, we determine that this transition is associated with gene and protein hallmarks of the epithelial-mesenchymal transition (EMT) in tumor cells, in particular upregulation of SNAIL and SLUG mRNA and loss of E-Cadherin protein. We identified the secreted proteins that arise from epithelial-fibroblast interactions in this system using an antibody-based array. The proteins secreted into the conditioned medium are sufficient to induce EMT markers in primary normal human mammary epithelial cells (HMECs). Our report demonstrates that estrogen receptor activity is dynamic and therefore is not best assessed with immunohistochemical techniques alone. For the facile and rapid determination of ER activity in a clinical setting, we propose the use of an emerging imaging method, highly sensitive label-free Fourier Transform infrared (FT-IR) spectroscopic imaging. We validate this technology in predicting endocrine sensitivity in breast cancer here, by detecting significant changes in spectroscopic peaks correlated with proliferation and metabolism of MCF7 cells grown in 3D culture.(Supported by grants from The Breast Cancer Research Foundation to BSK, DOD postdoctoral fellowship W81XWH-09-1-0398 to AB, NIH NCI Alliance for Nanotechnology in Cancer ‘Midwest Cancer Nanotechnology Training Center R25 CA154015A to SEH, and National Institutes of Health RO1CA138882 to RB.)Citation Format: Sarah E. Holton, Anna Bergamaschi, Benita S. Katzenellenbogen, Rohit Bhargava. Mammary fibroblasts induce hormone-independent growth in estrogen receptor-positive breast cancer cells via an epithelial-to-mesenchymal transition in a 3D cell culture model. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 503. doi:10.1158/1538-7445.AM2013-503" @default.
- W2020563079 created "2016-06-24" @default.
- W2020563079 creator A5023245938 @default.
- W2020563079 creator A5032157470 @default.
- W2020563079 creator A5042796903 @default.
- W2020563079 creator A5080531304 @default.
- W2020563079 date "2013-04-15" @default.
- W2020563079 modified "2023-09-25" @default.
- W2020563079 title "Abstract 503: Mammary fibroblasts induce hormone-independent growth in estrogen receptor-positive breast cancer cells via an epithelial-to-mesenchymal transition in a 3D cell culture model." @default.
- W2020563079 doi "https://doi.org/10.1158/1538-7445.am2013-503" @default.
- W2020563079 hasPublicationYear "2013" @default.
- W2020563079 type Work @default.
- W2020563079 sameAs 2020563079 @default.
- W2020563079 citedByCount "0" @default.
- W2020563079 crossrefType "proceedings-article" @default.
- W2020563079 hasAuthorship W2020563079A5023245938 @default.
- W2020563079 hasAuthorship W2020563079A5032157470 @default.
- W2020563079 hasAuthorship W2020563079A5042796903 @default.
- W2020563079 hasAuthorship W2020563079A5080531304 @default.
- W2020563079 hasConcept C121608353 @default.
- W2020563079 hasConcept C126322002 @default.
- W2020563079 hasConcept C134018914 @default.
- W2020563079 hasConcept C172313692 @default.
- W2020563079 hasConcept C2776187077 @default.
- W2020563079 hasConcept C2777164284 @default.
- W2020563079 hasConcept C2779013556 @default.
- W2020563079 hasConcept C502942594 @default.
- W2020563079 hasConcept C530470458 @default.
- W2020563079 hasConcept C71924100 @default.
- W2020563079 hasConcept C76419328 @default.
- W2020563079 hasConcept C84606932 @default.
- W2020563079 hasConcept C86803240 @default.
- W2020563079 hasConcept C96232424 @default.
- W2020563079 hasConcept C96307122 @default.
- W2020563079 hasConcept C98717036 @default.
- W2020563079 hasConceptScore W2020563079C121608353 @default.
- W2020563079 hasConceptScore W2020563079C126322002 @default.
- W2020563079 hasConceptScore W2020563079C134018914 @default.
- W2020563079 hasConceptScore W2020563079C172313692 @default.
- W2020563079 hasConceptScore W2020563079C2776187077 @default.
- W2020563079 hasConceptScore W2020563079C2777164284 @default.
- W2020563079 hasConceptScore W2020563079C2779013556 @default.
- W2020563079 hasConceptScore W2020563079C502942594 @default.
- W2020563079 hasConceptScore W2020563079C530470458 @default.
- W2020563079 hasConceptScore W2020563079C71924100 @default.
- W2020563079 hasConceptScore W2020563079C76419328 @default.
- W2020563079 hasConceptScore W2020563079C84606932 @default.
- W2020563079 hasConceptScore W2020563079C86803240 @default.
- W2020563079 hasConceptScore W2020563079C96232424 @default.
- W2020563079 hasConceptScore W2020563079C96307122 @default.
- W2020563079 hasConceptScore W2020563079C98717036 @default.
- W2020563079 hasLocation W20205630791 @default.
- W2020563079 hasOpenAccess W2020563079 @default.
- W2020563079 hasPrimaryLocation W20205630791 @default.
- W2020563079 hasRelatedWork W100013975 @default.
- W2020563079 hasRelatedWork W1500059225 @default.
- W2020563079 hasRelatedWork W1971013160 @default.
- W2020563079 hasRelatedWork W1982146272 @default.
- W2020563079 hasRelatedWork W1982922557 @default.
- W2020563079 hasRelatedWork W1984950084 @default.
- W2020563079 hasRelatedWork W2000642539 @default.
- W2020563079 hasRelatedWork W2021041130 @default.
- W2020563079 hasRelatedWork W2031038566 @default.
- W2020563079 hasRelatedWork W2054156840 @default.
- W2020563079 hasRelatedWork W2061549252 @default.
- W2020563079 hasRelatedWork W2140089334 @default.
- W2020563079 hasRelatedWork W2164179286 @default.
- W2020563079 hasRelatedWork W2323544206 @default.
- W2020563079 hasRelatedWork W2333269372 @default.
- W2020563079 hasRelatedWork W2739952712 @default.
- W2020563079 hasRelatedWork W2944800867 @default.
- W2020563079 hasRelatedWork W2956032049 @default.
- W2020563079 hasRelatedWork W3139596206 @default.
- W2020563079 hasRelatedWork W568544598 @default.
- W2020563079 isParatext "false" @default.
- W2020563079 isRetracted "false" @default.
- W2020563079 magId "2020563079" @default.
- W2020563079 workType "article" @default.