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- W2020565513 abstract "RNA polymerase holoenzymes from many bacterial species share a common promoter recognition specificity since they use the same promoter sites on a variety of templates. These promoters generally include sequences homologous to the average sequences -TTGACA- and -TATAATA-, located −35 and −10 base pairs, respectively, upstream of the transcriptional start site. We have isolated a minor form of B. subtilis RNA polymerase in which the normal sigma subunit (σ 55 ) is replaced by a smaller polypeptide (σ 28 ) and which is highly specific for a class of promoter sites not used by the σ 55 -holoenzyme. Sequencing of two B. subtilis promoter sites used by the σ 28 -holoenzyme reveals identical sequences at −35 and −10 base pairs from the start site, which are -CTAAA- and -CCGATAT-, respectively. These results confirm that sigma subunit plays a major direct role in promoter sequence recognition, and support a model in which sigma interacts sequentially with −35 and −10 regions, respectively." @default.
- W2020565513 created "2016-06-24" @default.
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- W2020565513 date "1981-01-01" @default.
- W2020565513 modified "2023-09-26" @default.
- W2020565513 title "Nucleotide sequences of two Bacillus subtilis promoters used by Bacillus subtilis sigma-28 RNA polymerase" @default.
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- W2020565513 doi "https://doi.org/10.1093/nar/9.22.5991" @default.
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