Matches in SemOpenAlex for { <https://semopenalex.org/work/W2020586682> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W2020586682 endingPage "2587" @default.
- W2020586682 startingPage "2587" @default.
- W2020586682 abstract "This work investigated the inducible gene activation in cancer cells that were sublethally injured during HIFU treatment. HeLa cells were transfected by an adenovirus vector that encodes GFP under the control of hsp70B promoter, leading to about 65% transfection efficiency. A volume of 10 μL transfected HeLa cells in suspension (5×107 cells/ml) were placed at the bottom of a PCR tube so that the cell suspension could be heated to a peak temperature of 50<th>°C, 60<th>°C, and 70<th>°C for 120, 10, and 1 s, respectively, by a focused 1.1-MHz HIFU transducer operated at a peak negative pressure of −2.7 MPa at different duty cycles. One day after HIFU treatment, cell viability was determined to be 63%, 35%, and 18%, respectively, based on Trypan Blue exclusion test. Importantly, in all test groups, inducible GFP expression was detected in about 40%–50% of the surviving cells with GFP intensity increased by 25-fold based on flow cytometry analysis. These results demonstrate that even under the short exposure duration of HIFU treatment, inducible gene expression could be produced in sublethally injured cell population in vitro. Further studies are underway to explore the optimal HIFU condition for gene activation in vivo." @default.
- W2020586682 created "2016-06-24" @default.
- W2020586682 creator A5018813756 @default.
- W2020586682 creator A5041441144 @default.
- W2020586682 creator A5052767668 @default.
- W2020586682 creator A5080492309 @default.
- W2020586682 date "2004-05-01" @default.
- W2020586682 modified "2023-09-27" @default.
- W2020586682 title "HIFU‐induced gene activation in vitro" @default.
- W2020586682 doi "https://doi.org/10.1121/1.4784368" @default.
- W2020586682 hasPublicationYear "2004" @default.
- W2020586682 type Work @default.
- W2020586682 sameAs 2020586682 @default.
- W2020586682 citedByCount "0" @default.
- W2020586682 crossrefType "journal-article" @default.
- W2020586682 hasAuthorship W2020586682A5018813756 @default.
- W2020586682 hasAuthorship W2020586682A5041441144 @default.
- W2020586682 hasAuthorship W2020586682A5052767668 @default.
- W2020586682 hasAuthorship W2020586682A5080492309 @default.
- W2020586682 hasConcept C104317684 @default.
- W2020586682 hasConcept C142613039 @default.
- W2020586682 hasConcept C1491633281 @default.
- W2020586682 hasConcept C150903083 @default.
- W2020586682 hasConcept C153911025 @default.
- W2020586682 hasConcept C185592680 @default.
- W2020586682 hasConcept C202751555 @default.
- W2020586682 hasConcept C207001950 @default.
- W2020586682 hasConcept C2777366897 @default.
- W2020586682 hasConcept C2780070996 @default.
- W2020586682 hasConcept C2908647359 @default.
- W2020586682 hasConcept C53227056 @default.
- W2020586682 hasConcept C54009773 @default.
- W2020586682 hasConcept C54355233 @default.
- W2020586682 hasConcept C553184892 @default.
- W2020586682 hasConcept C55493867 @default.
- W2020586682 hasConcept C71924100 @default.
- W2020586682 hasConcept C81885089 @default.
- W2020586682 hasConcept C86803240 @default.
- W2020586682 hasConcept C99454951 @default.
- W2020586682 hasConceptScore W2020586682C104317684 @default.
- W2020586682 hasConceptScore W2020586682C142613039 @default.
- W2020586682 hasConceptScore W2020586682C1491633281 @default.
- W2020586682 hasConceptScore W2020586682C150903083 @default.
- W2020586682 hasConceptScore W2020586682C153911025 @default.
- W2020586682 hasConceptScore W2020586682C185592680 @default.
- W2020586682 hasConceptScore W2020586682C202751555 @default.
- W2020586682 hasConceptScore W2020586682C207001950 @default.
- W2020586682 hasConceptScore W2020586682C2777366897 @default.
- W2020586682 hasConceptScore W2020586682C2780070996 @default.
- W2020586682 hasConceptScore W2020586682C2908647359 @default.
- W2020586682 hasConceptScore W2020586682C53227056 @default.
- W2020586682 hasConceptScore W2020586682C54009773 @default.
- W2020586682 hasConceptScore W2020586682C54355233 @default.
- W2020586682 hasConceptScore W2020586682C553184892 @default.
- W2020586682 hasConceptScore W2020586682C55493867 @default.
- W2020586682 hasConceptScore W2020586682C71924100 @default.
- W2020586682 hasConceptScore W2020586682C81885089 @default.
- W2020586682 hasConceptScore W2020586682C86803240 @default.
- W2020586682 hasConceptScore W2020586682C99454951 @default.
- W2020586682 hasIssue "5_Supplement" @default.
- W2020586682 hasLocation W20205866821 @default.
- W2020586682 hasOpenAccess W2020586682 @default.
- W2020586682 hasPrimaryLocation W20205866821 @default.
- W2020586682 hasRelatedWork W1761168594 @default.
- W2020586682 hasRelatedWork W2047043835 @default.
- W2020586682 hasRelatedWork W2073729403 @default.
- W2020586682 hasRelatedWork W2080787715 @default.
- W2020586682 hasRelatedWork W2349069328 @default.
- W2020586682 hasRelatedWork W2357517417 @default.
- W2020586682 hasRelatedWork W2365174857 @default.
- W2020586682 hasRelatedWork W2379416539 @default.
- W2020586682 hasRelatedWork W2381695351 @default.
- W2020586682 hasRelatedWork W2386797884 @default.
- W2020586682 hasVolume "115" @default.
- W2020586682 isParatext "false" @default.
- W2020586682 isRetracted "false" @default.
- W2020586682 magId "2020586682" @default.
- W2020586682 workType "article" @default.