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- W2020648411 abstract "We have recently developed a wide-field photon-counting detector having high-temporal and high-spatial resolutions and capable of high-throughput (the H33D detector). Its design is based on a 25 mm diameter multi-alkali photocathode producing one photo electron per detected photon, which are then multiplied up to 10<sup>7</sup> times by a 3-microchannel plate stack. The resulting electron cloud is proximity focused on a cross delay line anode, which allows determining the incident photon position with high accuracy. The imaging and fluorescence lifetime measurement performances of the H33D detector installed on a standard epifluorescence microscope will be presented. We compare them to those of standard single-molecule detectors such as single-photon avalanche photodiode (SPAD) or electron-multiplying camera using model samples (fluorescent beads, quantum dots and live cells). Finally, we discuss the design and applications of future generation of H33D detectors for single-molecule imaging and high-throughput study of biomolecular interactions." @default.
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- W2020648411 date "2006-02-09" @default.
- W2020648411 modified "2023-10-17" @default.
- W2020648411 title "A space- and time-resolved single photon counting detector for fluorescence microscopy and spectroscopy" @default.
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- W2020648411 doi "https://doi.org/10.1117/12.646482" @default.
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