Matches in SemOpenAlex for { <https://semopenalex.org/work/W2020656010> ?p ?o ?g. }
- W2020656010 abstract "Isotopic substitution provides an effective tool to probe the mechanism of enzyme-catalyzed reactions. To our knowledge, kinetic isotope effects on the enantioselectivity of enzymes have not been reported. We investigated the effect of deuterium substitution on the enantiomeric ratio, E, of PQQ-containing quinohemoprotein alcohol dehydrogenase, QH-ADH, from Comamonas testosteroni in the ferricyanide-coupled kinetic resolution of rac-2,2-dimethyl-4-hydroxymethyl-1,3-dioxolane, solketal. Under otherwise identical conditions, we measured E=30 for solketal and E=6 for rac-2,2-dimethyl-4-[1,1-2H]hydroxymethyl-1,3-[5,5,4-2H]dioxolane, d(5)-solketal. It is proposed that isotopic substitution affects the relative kinetic weights of the initial hydron/deuteron transfer from substrate to cofactor and the subsequent proton/deuteron shift in the cofactor-product complex. The latter step becomes more important in the deuterated complex to the extent that the enantiomer discrimination in the first step is partially overruled." @default.
- W2020656010 created "2016-06-24" @default.
- W2020656010 creator A5005699911 @default.
- W2020656010 creator A5008826703 @default.
- W2020656010 creator A5018150139 @default.
- W2020656010 date "2003-04-01" @default.
- W2020656010 modified "2023-09-25" @default.
- W2020656010 title "Deuterium isotope effect on enantioselectivity in the Comamonas testosteroni quinohemoprotein alcohol dehydrogenase-catalyzed kinetic resolution of rac-2,2-dimethyl-4-hydroxymethyl-1,3-dioxolane, solketal" @default.
- W2020656010 cites W1595715800 @default.
- W2020656010 cites W1601946006 @default.
- W2020656010 cites W1942629826 @default.
- W2020656010 cites W1971420248 @default.
- W2020656010 cites W1973392547 @default.
- W2020656010 cites W1975331893 @default.
- W2020656010 cites W1982453968 @default.
- W2020656010 cites W1983418889 @default.
- W2020656010 cites W1989043748 @default.
- W2020656010 cites W1996054932 @default.
- W2020656010 cites W1998536306 @default.
- W2020656010 cites W1999132310 @default.
- W2020656010 cites W2003871110 @default.
- W2020656010 cites W2005470705 @default.
- W2020656010 cites W2008410493 @default.
- W2020656010 cites W2010622037 @default.
- W2020656010 cites W2016540344 @default.
- W2020656010 cites W2019508111 @default.
- W2020656010 cites W2021988071 @default.
- W2020656010 cites W2024581675 @default.
- W2020656010 cites W2026117532 @default.
- W2020656010 cites W2030471513 @default.
- W2020656010 cites W2032150516 @default.
- W2020656010 cites W2037740275 @default.
- W2020656010 cites W2046586066 @default.
- W2020656010 cites W2060790233 @default.
- W2020656010 cites W2066029781 @default.
- W2020656010 cites W2069300531 @default.
- W2020656010 cites W2076656955 @default.
- W2020656010 cites W2078133320 @default.
- W2020656010 cites W2080100849 @default.
- W2020656010 cites W2086185283 @default.
- W2020656010 cites W2091002165 @default.
- W2020656010 cites W2100770832 @default.
- W2020656010 cites W2103659902 @default.
- W2020656010 cites W2129917615 @default.
- W2020656010 cites W2152319453 @default.
- W2020656010 cites W2154689668 @default.
- W2020656010 cites W2157010946 @default.
- W2020656010 cites W2157033822 @default.
- W2020656010 cites W2189785354 @default.
- W2020656010 cites W2281766802 @default.
- W2020656010 cites W2418894567 @default.
- W2020656010 cites W2950573934 @default.
- W2020656010 cites W2952788042 @default.
- W2020656010 doi "https://doi.org/10.1016/s1570-9639(03)00073-6" @default.
- W2020656010 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12686148" @default.
- W2020656010 hasPublicationYear "2003" @default.
- W2020656010 type Work @default.
- W2020656010 sameAs 2020656010 @default.
- W2020656010 citedByCount "2" @default.
- W2020656010 countsByYear W20206560102013 @default.
- W2020656010 countsByYear W20206560102015 @default.
- W2020656010 crossrefType "journal-article" @default.
- W2020656010 hasAuthorship W2020656010A5005699911 @default.
- W2020656010 hasAuthorship W2020656010A5008826703 @default.
- W2020656010 hasAuthorship W2020656010A5018150139 @default.
- W2020656010 hasConcept C121332964 @default.
- W2020656010 hasConcept C146686406 @default.
- W2020656010 hasConcept C161790260 @default.
- W2020656010 hasConcept C178790620 @default.
- W2020656010 hasConcept C181199279 @default.
- W2020656010 hasConcept C185592680 @default.
- W2020656010 hasConcept C197957613 @default.
- W2020656010 hasConcept C2776666804 @default.
- W2020656010 hasConcept C2777344606 @default.
- W2020656010 hasConcept C2780021053 @default.
- W2020656010 hasConcept C2780591741 @default.
- W2020656010 hasConcept C2781066024 @default.
- W2020656010 hasConcept C28173026 @default.
- W2020656010 hasConcept C486523 @default.
- W2020656010 hasConcept C58364064 @default.
- W2020656010 hasConcept C62520636 @default.
- W2020656010 hasConcept C69928629 @default.
- W2020656010 hasConcept C71240020 @default.
- W2020656010 hasConceptScore W2020656010C121332964 @default.
- W2020656010 hasConceptScore W2020656010C146686406 @default.
- W2020656010 hasConceptScore W2020656010C161790260 @default.
- W2020656010 hasConceptScore W2020656010C178790620 @default.
- W2020656010 hasConceptScore W2020656010C181199279 @default.
- W2020656010 hasConceptScore W2020656010C185592680 @default.
- W2020656010 hasConceptScore W2020656010C197957613 @default.
- W2020656010 hasConceptScore W2020656010C2776666804 @default.
- W2020656010 hasConceptScore W2020656010C2777344606 @default.
- W2020656010 hasConceptScore W2020656010C2780021053 @default.
- W2020656010 hasConceptScore W2020656010C2780591741 @default.
- W2020656010 hasConceptScore W2020656010C2781066024 @default.
- W2020656010 hasConceptScore W2020656010C28173026 @default.
- W2020656010 hasConceptScore W2020656010C486523 @default.
- W2020656010 hasConceptScore W2020656010C58364064 @default.
- W2020656010 hasConceptScore W2020656010C62520636 @default.
- W2020656010 hasConceptScore W2020656010C69928629 @default.