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- W2020661838 abstract "Monoclonal antibody from rat hybridoma cell line ARC MAC 50.1 binds more strongly to Pfr than to Pr in a sandwich ELISA of phytochrome in crude Avena sativa L. extracts or highly purified Avena phytochrome. Discrimination is not a consequence of differential modification of Pr and Pfr during the assay. Loss of a 6 kDa peptide from the N-terminal end of the phytochrome apoprotein during purification does not prevent discrimination. Neither is preferential binding to Pfr a consequence of a steric interaction between ARC MAC 50.1 and the antibody used with it in the sandwich ELISA. It is concluded that the binding site for ARC MAC 50.1 undergoes a reversible conformational change upon photoconversion and may thus represent a functional region of the phytochrome molecule." @default.
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- W2020661838 modified "2023-09-25" @default.
- W2020661838 title "Monoclonal antibody ARC MAC 50.1 binds to a site on the phytochrome molecule which undergoes a photoreversible conformational change" @default.
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