FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2020663376> ?p ?o ?g. }

• W2020663376 endingPage "149" @default.
• W2020663376 startingPage "143" @default.
• W2020663376 abstract "A detergent-solubilized fraction of skin mucus of carp (Cyprinus carpio) induced ion channels after reconstitution into planar lipid bilayers. A differential extraction using a non-ionic detergent followed by electrophoretic separation led to the isolation of two hydrophobic 31-kDa and 27-kDa proteins. In contrast to the 27-kDa protein, which was glycosylated, the 31-kDa did not bind to concanavalin A. The reconstitution of these proteins into a planar lipid bilayer restored the ionophore behavior already observed with the crude mucus. The main unit conductance levels were about 900 pS for the 27-kDa protein and 500 pS for the 31-kDa protein, and selectivity measurements gave PCl/PK ratios of 0.6 and 1.0, respectively. These proteins had large potent microbicidal activities (0.018–0.18 μM) against different strains of gram-negative and gram-positive bacteria. This behavior can be compared with insect defensins that are known to form large ion channels in the bacterial membrane. To exclude the eventuality of bacterial origin, the bacterial flora of the crude mucus were analysed and the following were identified: Pseudomonas cepacia; Micrococcus luteus; Micrococcus roseus; Flavobacterium sp.; Aeromonas hydrophila. Antibacterial assays with both proteins were performed against these specific strains and revealed good growth inhibition activities. Furthermore, microsequencing analysis showed that the 31-kDa protein was protected on its N-terminal extremity in contrast to the 27-kDa protein, which had a 19-amino-acid sequence. This last sequence, when compared with sequences in protein data banks, did not reveal any significant similarities to other proteins. These results suggest that these novel proteins could be involved in antibacterial defense processes in fish." @default.
• W2020663376 created "2016-06-24" @default.
• W2020663376 creator A5036305027 @default.
• W2020663376 creator A5059966003 @default.
• W2020663376 creator A5062545741 @default.
• W2020663376 creator A5063536320 @default.
• W2020663376 creator A5066685728 @default.
• W2020663376 creator A5087763241 @default.
• W2020663376 date "1996-08-01" @default.
• W2020663376 modified "2023-09-26" @default.
• W2020663376 title "Characterization and Ion Channel Activities of Novel Antibacterial Proteins from the Skin Mucosa of Carp (<i>Cyprinus carpio</i>)" @default.
• W2020663376 cites W1502716368 @default.
• W2020663376 cites W1648158953 @default.
• W2020663376 cites W1970621669 @default.
• W2020663376 cites W1982683980 @default.
• W2020663376 cites W1988901585 @default.
• W2020663376 cites W1992786825 @default.
• W2020663376 cites W1996652558 @default.
• W2020663376 cites W2000562563 @default.
• W2020663376 cites W2006211522 @default.
• W2020663376 cites W2014181077 @default.
• W2020663376 cites W2018309785 @default.
• W2020663376 cites W2034348397 @default.
• W2020663376 cites W2041521712 @default.
• W2020663376 cites W2045871462 @default.
• W2020663376 cites W2053026623 @default.
• W2020663376 cites W2063159663 @default.
• W2020663376 cites W2068620810 @default.
• W2020663376 cites W2072756034 @default.
• W2020663376 cites W2072933784 @default.
• W2020663376 cites W2079468421 @default.
• W2020663376 cites W2084190681 @default.
• W2020663376 cites W2085483097 @default.
• W2020663376 cites W2085732324 @default.
• W2020663376 cites W2097400197 @default.
• W2020663376 cites W2100837269 @default.
• W2020663376 cites W2170528669 @default.
• W2020663376 cites W2494179532 @default.
• W2020663376 cites W38787200 @default.
• W2020663376 cites W4239212854 @default.
• W2020663376 cites W4295744777 @default.
• W2020663376 doi "https://doi.org/10.1111/j.1432-1033.1996.0143h.x" @default.
• W2020663376 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8797847" @default.
• W2020663376 hasPublicationYear "1996" @default.
• W2020663376 type Work @default.
• W2020663376 sameAs 2020663376 @default.
• W2020663376 citedByCount "105" @default.
• W2020663376 countsByYear W20206633762012 @default.
• W2020663376 countsByYear W20206633762013 @default.
• W2020663376 countsByYear W20206633762014 @default.
• W2020663376 countsByYear W20206633762015 @default.
• W2020663376 countsByYear W20206633762016 @default.
• W2020663376 countsByYear W20206633762017 @default.
• W2020663376 countsByYear W20206633762018 @default.
• W2020663376 countsByYear W20206633762019 @default.
• W2020663376 countsByYear W20206633762021 @default.
• W2020663376 countsByYear W20206633762022 @default.
• W2020663376 countsByYear W20206633762023 @default.
• W2020663376 crossrefType "journal-article" @default.
• W2020663376 hasAuthorship W2020663376A5036305027 @default.
• W2020663376 hasAuthorship W2020663376A5059966003 @default.
• W2020663376 hasAuthorship W2020663376A5062545741 @default.
• W2020663376 hasAuthorship W2020663376A5063536320 @default.
• W2020663376 hasAuthorship W2020663376A5066685728 @default.
• W2020663376 hasAuthorship W2020663376A5087763241 @default.
• W2020663376 hasBestOaLocation W20206633761 @default.
• W2020663376 hasConcept C104317684 @default.
• W2020663376 hasConcept C18903297 @default.
• W2020663376 hasConcept C2776683811 @default.
• W2020663376 hasConcept C2777312293 @default.
• W2020663376 hasConcept C2778106697 @default.
• W2020663376 hasConcept C2781463711 @default.
• W2020663376 hasConcept C523546767 @default.
• W2020663376 hasConcept C54355233 @default.
• W2020663376 hasConcept C547475151 @default.
• W2020663376 hasConcept C55493867 @default.
• W2020663376 hasConcept C86803240 @default.
• W2020663376 hasConcept C89423630 @default.
• W2020663376 hasConceptScore W2020663376C104317684 @default.
• W2020663376 hasConceptScore W2020663376C18903297 @default.
• W2020663376 hasConceptScore W2020663376C2776683811 @default.
• W2020663376 hasConceptScore W2020663376C2777312293 @default.
• W2020663376 hasConceptScore W2020663376C2778106697 @default.
• W2020663376 hasConceptScore W2020663376C2781463711 @default.
• W2020663376 hasConceptScore W2020663376C523546767 @default.
• W2020663376 hasConceptScore W2020663376C54355233 @default.
• W2020663376 hasConceptScore W2020663376C547475151 @default.
• W2020663376 hasConceptScore W2020663376C55493867 @default.
• W2020663376 hasConceptScore W2020663376C86803240 @default.
• W2020663376 hasConceptScore W2020663376C89423630 @default.
• W2020663376 hasIssue "1" @default.
• W2020663376 hasLocation W20206633761 @default.
• W2020663376 hasLocation W20206633762 @default.
• W2020663376 hasLocation W20206633763 @default.
• W2020663376 hasOpenAccess W2020663376 @default.
• W2020663376 hasPrimaryLocation W20206633761 @default.
• W2020663376 hasRelatedWork W1540968790 @default.
• W2020663376 hasRelatedWork W1920550561 @default.

• next