FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2020665587> ?p ?o ?g. }

• W2020665587 endingPage "11554" @default.
• W2020665587 startingPage "11544" @default.
• W2020665587 abstract "The Rous sarcoma virus (RSV) transmembrane (TM) glycoprotein is modified by the addition of palmitic acid. To identify whether conserved cysteines within the hydrophobic anchor region are the site(s) of palmitoylation, and to determine the role of acylation in glycoprotein function, cysteines at residues 164 and 167 of the TM protein were mutated to glycine (C164G, C167G, and C164G/C167G). In CV-1 cells, palmitate was added to env gene products containing single mutations but was absent in the double-mutant Env. Although mutant Pr95 Env precursors were synthesized with wild-type kinetics, the phenotypes of the mutants differed markedly. Env-C164G had properties similar to those of the wild type, while Env-C167G was degraded faster, and Env containing the double mutant C164G/C167G was very rapidly degraded. Degradation occurred after transient plasma membrane expression. The decrease in steady-state surface expression and increased rate of internalization into endosomes and lysosomes paralleled the decrease in palmitoylation observed for the mutants. The phenotypes of mutant viruses were assessed in avian cells in the context of the pATV8R proviral genome. Virus containing the C164G mutation replicated with wild-type kinetics but exhibited reduced peak reverse transcriptase levels. In contrast, viruses containing either the C167G or the C164G/C167G mutation were poorly infectious or noninfectious, respectively. These phenotypes correlated with different degrees of glycoprotein incorporation into virions. Infectious revertants of the double mutant demonstrated the importance of cysteine-167 for efficient plasma membrane expression and Env incorporation. The observation that both cysteines within the membrane-spanning domain are accessible for acylation has implications for the topology of this region, and a model is proposed." @default.
• W2020665587 created "2016-06-24" @default.
• W2020665587 creator A5020075616 @default.
• W2020665587 creator A5030433049 @default.
• W2020665587 creator A5035881745 @default.
• W2020665587 creator A5062604430 @default.
• W2020665587 creator A5090911204 @default.
• W2020665587 date "2001-12-01" @default.
• W2020665587 modified "2023-09-25" @default.
• W2020665587 title "Palmitoylation of the Rous Sarcoma Virus Transmembrane Glycoprotein Is Required for Protein Stability and Virus Infectivity" @default.
• W2020665587 cites W1481094350 @default.
• W2020665587 cites W1487090760 @default.
• W2020665587 cites W1496756293 @default.
• W2020665587 cites W1505416249 @default.
• W2020665587 cites W1512128997 @default.
• W2020665587 cites W1556716938 @default.
• W2020665587 cites W1564144670 @default.
• W2020665587 cites W1567879434 @default.
• W2020665587 cites W158060014 @default.
• W2020665587 cites W1585000618 @default.
• W2020665587 cites W1593943572 @default.
• W2020665587 cites W1635142346 @default.
• W2020665587 cites W1700316245 @default.
• W2020665587 cites W1833064266 @default.
• W2020665587 cites W1849510551 @default.
• W2020665587 cites W1953927339 @default.
• W2020665587 cites W1966998035 @default.
• W2020665587 cites W1976293601 @default.
• W2020665587 cites W1977553139 @default.
• W2020665587 cites W1978796303 @default.
• W2020665587 cites W1983926699 @default.
• W2020665587 cites W1992551509 @default.
• W2020665587 cites W1995097201 @default.
• W2020665587 cites W1998865212 @default.
• W2020665587 cites W2005340946 @default.
• W2020665587 cites W2009599622 @default.
• W2020665587 cites W2010830658 @default.
• W2020665587 cites W2015943185 @default.
• W2020665587 cites W2016417523 @default.
• W2020665587 cites W2029028351 @default.
• W2020665587 cites W2034277598 @default.
• W2020665587 cites W2035317074 @default.
• W2020665587 cites W2035706870 @default.
• W2020665587 cites W2042203458 @default.
• W2020665587 cites W2045883968 @default.
• W2020665587 cites W2051566412 @default.
• W2020665587 cites W2053031082 @default.
• W2020665587 cites W2063301738 @default.
• W2020665587 cites W2066573335 @default.
• W2020665587 cites W2067691363 @default.
• W2020665587 cites W2068385155 @default.
• W2020665587 cites W2070734260 @default.
• W2020665587 cites W2075622704 @default.
• W2020665587 cites W2077077183 @default.
• W2020665587 cites W2077745420 @default.
• W2020665587 cites W2079340910 @default.
• W2020665587 cites W2080543241 @default.
• W2020665587 cites W2082861222 @default.
• W2020665587 cites W2092296126 @default.
• W2020665587 cites W2105710739 @default.
• W2020665587 cites W2121936687 @default.
• W2020665587 cites W2133388060 @default.
• W2020665587 cites W2135380694 @default.
• W2020665587 cites W2139846514 @default.
• W2020665587 cites W2153377782 @default.
• W2020665587 cites W2168404339 @default.
• W2020665587 cites W2170478363 @default.
• W2020665587 cites W2170549914 @default.
• W2020665587 cites W2288402731 @default.
• W2020665587 cites W2464624046 @default.
• W2020665587 cites W2489566220 @default.
• W2020665587 cites W4243378881 @default.
• W2020665587 doi "https://doi.org/10.1128/jvi.75.23.11544-11554.2001" @default.
• W2020665587 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/114741" @default.
• W2020665587 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11689636" @default.
• W2020665587 hasPublicationYear "2001" @default.
• W2020665587 type Work @default.
• W2020665587 sameAs 2020665587 @default.
• W2020665587 citedByCount "38" @default.
• W2020665587 countsByYear W20206655872012 @default.
• W2020665587 countsByYear W20206655872013 @default.
• W2020665587 countsByYear W20206655872016 @default.
• W2020665587 countsByYear W20206655872018 @default.
• W2020665587 countsByYear W20206655872020 @default.
• W2020665587 countsByYear W20206655872021 @default.
• W2020665587 crossrefType "journal-article" @default.
• W2020665587 hasAuthorship W2020665587A5020075616 @default.
• W2020665587 hasAuthorship W2020665587A5030433049 @default.
• W2020665587 hasAuthorship W2020665587A5035881745 @default.
• W2020665587 hasAuthorship W2020665587A5062604430 @default.
• W2020665587 hasAuthorship W2020665587A5090911204 @default.
• W2020665587 hasBestOaLocation W20206655872 @default.
• W2020665587 hasConcept C104317684 @default.
• W2020665587 hasConcept C108625454 @default.
• W2020665587 hasConcept C118892022 @default.
• W2020665587 hasConcept C143065580 @default.
• W2020665587 hasConcept C146727910 @default.
• W2020665587 hasConcept C153911025 @default.

• next