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- W2020682095 abstract "Colon cancer is the second most common malignant disease in Hungary. The most frequently used drug is 5-fluorouracil, which has no effect in the 90% of the cases. The main mechanisms of the resistance against 5-fluorouracil are due to the overexpression of dihydropyrimidine dehydrogenase, B p65. The investigation of these single markers κMRP8, timidilate synthetase, NF has not yet provided clinically useful results, thus the simultaneous investigation of multiple markers is needed. DNA microarrays provide the best available technology for this purpose. Based on previous publications, we have set up a consensus gene list (n=39), containing genes associated with resistance against 5-fluorouracil in multiple studies. We have constructed a custom DNA chip containing these genes and 4 housekeeping control genes. We measured the expression of all genes in quadruplicate and computed the relative expression compared to the housekeeping genes. We have collected 70 colon cancer clinical samples in the Uzsoki Hospital. We have isolated RNA using Qiagen RNeasy mini kit, the quality of the samples was measured on a 1% gelelectrophoresis and the quantity by Nanodrop technology. The hybridization to the arrays was performed using the Genisphere Array MPX kit. Statistical evaluation was performed using student's t-test with Bonferroni correction. The results indicate no difference in the mechanism of resistance comparing oral and intravenous 5-fluorouracil. Summarizing our results we have developed a consensus microarray to predict 5-fluorouracil resistance in colon cancer. While preliminary results show the feasibility of the technology, currently the extension to a large cohort of additional patients is reroute." @default.
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- W2020682095 date "2007-05-01" @default.
- W2020682095 modified "2023-10-18" @default.
- W2020682095 title "Diagnostic gene expression signatures to predict the chemotherapy resistance against oral and intravenous 5-fluorouracil" @default.
- W2020682095 doi "https://doi.org/10.1055/s-2007-982694" @default.
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