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• W2020684712 abstract "The molecular interactions between sialoadhesin and sialylated ligands have been investigated by using proton NMR. Addition of ligands to the 12 kDa N-terminal immunoglobulin-like domain of sialoadhesin result in resonance shifts in the protein spectrum that have been used to determine the affinities of sialoadhesin for several sialosides. The results indicate that α2,3-sialyl-lactose and α2,6-sialyl-lactose bind respectively 2- and 1.5-fold more strongly than does α-methyl-N-acetylneuraminic acid (α-Me-NeuAc). The resonances corresponding to the methyl protons within the N-acetyl moiety of sialic acid undergo upfield shifting and broadening during titrations, reflecting an interaction of this group with Trp2 in sialoadhesin as observed in co-crystals of the terminal domain with bound ligand. This resonance shift was used to measure the affinities of mutant and wild-type forms of sialoadhesin in which the first three domains are fused to the Fc region of human IgG1. Substitution of Arg97 by alanine completely abrogated measurable interaction with α-Me-NeuAc, whereas a conservative substitution with lysine resulted in a 10-fold decrease in affinity. These results provide the first direct measurement of the affinity of sialoadhesin for sialosides and confirm the critical importance of the conserved arginine in interactions between sialosides and members of the siglec family of sialic acid-binding, immunoglobulin-like lectins." @default.
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• W2020684712 date "1999-07-08" @default.
• W2020684712 modified "2023-10-18" @default.
• W2020684712 title "Molecular analysis of sialoside binding to sialoadhesin by NMR and site-directed mutagenesis" @default.
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• W2020684712 doi "https://doi.org/10.1042/bj3410355" @default.
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