FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2020735206> ?p ?o ?g. }

• W2020735206 endingPage "433" @default.
• W2020735206 startingPage "421" @default.
• W2020735206 abstract "Among the Trichinella TSL-1 antigens, whose antigenicity is generally due mainly to tyvelose-containing epitopes, gp53 is unusual in that its antigenicity is due mainly to protein epitopes. In the present study we mapped two of these epitopes, recognized by monoclonal antibodies (mAbs) that specifically recognize gp53 from all encysting Trichinella species (mAb US9), or gp53 from Trichinella spiralis alone (mAb US5). Based on previously published sequences of this glycoprotein [Mol. Biochem. Parasitol. 72 (1995) 253], in this study, we cloned the full gp53 cDNA from a new strain, Trichinella britovi (ISS 11; AN: ), and from another T. spiralis isolate (ISS 115; AN: ). The gp53 sequence comprised an ORF of 1239bp, coding for 412 amino acids, with 61 nucleotide differences (resulting in 38 residue changes) between the two species. Mapping of US5- and US9-recognized epitopes was undertaken through the construction and expression in the pGEX4T vector of truncated gp53 peptides, and by the construction of peptides derived from the antigenic regions. The epitope recognized by mAb US9 was a linear peptide of 8 residues, 33Met- 40Ser, located in the amino-terminal region, while the corresponding epitope recognized by mAb US5 was a 47-amino acid sequence containing two alpha-helix regions flanked by random coils, 290Thr- 336Lys. Molecular modeling of these peptides seems to indicate that recognition of the US9 epitope depends on the presence of two available hydroxyl groups provided by one methionine and one serine on T. spiralis gp53 (not present on Trichinella pseudospiralis gp53). Additionally, the stability of the US5 epitope seems to depend on correct folding of the 47-amino acid sequence (only present in T. spiralis). The relevance of these findings for understanding the antigenic recognition of Trichinella TSL-1 antigens, and for further studies to investigate possible function(s) of gp53 in Trichinella, is discussed." @default.
• W2020735206 created "2016-06-24" @default.
• W2020735206 creator A5006634749 @default.
• W2020735206 creator A5027320624 @default.
• W2020735206 creator A5035119690 @default.
• W2020735206 creator A5040363277 @default.
• W2020735206 creator A5060553694 @default.
• W2020735206 creator A5076272487 @default.
• W2020735206 creator A5082299315 @default.
• W2020735206 date "2004-06-01" @default.
• W2020735206 modified "2023-09-26" @default.
• W2020735206 title "Minor interspecies variations in the sequence of the gp53 TSL-1 antigen of Trichinella define species-specific immunodominant epitopes1" @default.
• W2020735206 cites W1540614756 @default.
• W2020735206 cites W1594700207 @default.
• W2020735206 cites W1604369171 @default.
• W2020735206 cites W1775749144 @default.
• W2020735206 cites W1978737089 @default.
• W2020735206 cites W1985645278 @default.
• W2020735206 cites W1991189282 @default.
• W2020735206 cites W2000628767 @default.
• W2020735206 cites W2021456198 @default.
• W2020735206 cites W2027110064 @default.
• W2020735206 cites W2044589156 @default.
• W2020735206 cites W2045174523 @default.
• W2020735206 cites W2053621743 @default.
• W2020735206 cites W2054928155 @default.
• W2020735206 cites W2062830151 @default.
• W2020735206 cites W2100837269 @default.
• W2020735206 cites W2101108802 @default.
• W2020735206 cites W2117019496 @default.
• W2020735206 cites W2122236473 @default.
• W2020735206 cites W2133374840 @default.
• W2020735206 cites W2137333216 @default.
• W2020735206 cites W2144638967 @default.
• W2020735206 cites W2153183731 @default.
• W2020735206 cites W2162113516 @default.
• W2020735206 cites W2169632705 @default.
• W2020735206 cites W2171091522 @default.
• W2020735206 cites W2330942048 @default.
• W2020735206 cites W2430220658 @default.
• W2020735206 cites W265936979 @default.
• W2020735206 cites W914119538 @default.
• W2020735206 doi "https://doi.org/10.1016/j.molimm.2004.03.032" @default.
• W2020735206 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15163539" @default.
• W2020735206 hasPublicationYear "2004" @default.
• W2020735206 type Work @default.
• W2020735206 sameAs 2020735206 @default.
• W2020735206 citedByCount "10" @default.
• W2020735206 countsByYear W20207352062016 @default.
• W2020735206 countsByYear W20207352062017 @default.
• W2020735206 countsByYear W20207352062019 @default.
• W2020735206 countsByYear W20207352062021 @default.
• W2020735206 crossrefType "journal-article" @default.
• W2020735206 hasAuthorship W2020735206A5006634749 @default.
• W2020735206 hasAuthorship W2020735206A5027320624 @default.
• W2020735206 hasAuthorship W2020735206A5035119690 @default.
• W2020735206 hasAuthorship W2020735206A5040363277 @default.
• W2020735206 hasAuthorship W2020735206A5060553694 @default.
• W2020735206 hasAuthorship W2020735206A5076272487 @default.
• W2020735206 hasAuthorship W2020735206A5082299315 @default.
• W2020735206 hasConcept C104317684 @default.
• W2020735206 hasConcept C147483822 @default.
• W2020735206 hasConcept C153911025 @default.
• W2020735206 hasConcept C159654299 @default.
• W2020735206 hasConcept C165901193 @default.
• W2020735206 hasConcept C167625842 @default.
• W2020735206 hasConcept C195616568 @default.
• W2020735206 hasConcept C203014093 @default.
• W2020735206 hasConcept C2777581317 @default.
• W2020735206 hasConcept C2779026604 @default.
• W2020735206 hasConcept C2781320125 @default.
• W2020735206 hasConcept C515207424 @default.
• W2020735206 hasConcept C542903549 @default.
• W2020735206 hasConcept C54355233 @default.
• W2020735206 hasConcept C55493867 @default.
• W2020735206 hasConcept C86803240 @default.
• W2020735206 hasConceptScore W2020735206C104317684 @default.
• W2020735206 hasConceptScore W2020735206C147483822 @default.
• W2020735206 hasConceptScore W2020735206C153911025 @default.
• W2020735206 hasConceptScore W2020735206C159654299 @default.
• W2020735206 hasConceptScore W2020735206C165901193 @default.
• W2020735206 hasConceptScore W2020735206C167625842 @default.
• W2020735206 hasConceptScore W2020735206C195616568 @default.
• W2020735206 hasConceptScore W2020735206C203014093 @default.
• W2020735206 hasConceptScore W2020735206C2777581317 @default.
• W2020735206 hasConceptScore W2020735206C2779026604 @default.
• W2020735206 hasConceptScore W2020735206C2781320125 @default.
• W2020735206 hasConceptScore W2020735206C515207424 @default.
• W2020735206 hasConceptScore W2020735206C542903549 @default.
• W2020735206 hasConceptScore W2020735206C54355233 @default.
• W2020735206 hasConceptScore W2020735206C55493867 @default.
• W2020735206 hasConceptScore W2020735206C86803240 @default.
• W2020735206 hasIssue "4" @default.
• W2020735206 hasLocation W20207352061 @default.
• W2020735206 hasLocation W20207352062 @default.
• W2020735206 hasOpenAccess W2020735206 @default.
• W2020735206 hasPrimaryLocation W20207352061 @default.
• W2020735206 hasRelatedWork W1978737089 @default.

• next