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- W2020744633 abstract "Drosophila RNA polymerases I & II were used to transcribe a recombinant bacterial plasmid containing one copy of Drosophila ribosomal DNA. Both supercoiled and relaxed, closed circular plasmids were used. With Mg +2 as the divalent cation, enzyme I is much more active on both forms of the plasmid; the relaxed form in particular supports almost no RNA synthesis by enzyme II. When Mn +2 is present, differences in template efficiencies are minimal. The differences observed in the absence of Mn +2 seem to depend only on different preferences for the physical state of the template and not on recognition of specific promotor sequences, since enzyme I shows no strand selection when transcribing these plasmids." @default.
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- W2020744633 title "In vitro transcription of drosophila ribosomal genes using Drosophila RNA polymerases" @default.
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