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- W2020755524 abstract "Pseudomonas corrugata 28 is a Cr(VI)-hyper-resistant bacterium. A Cr(VI)-sensitive mutant was obtained by insertional mutagenesis using EZ-Tn5 <R6Kgammaori/KAN-2>Tnp. The mutant strain was impaired in a gene, here named oscA (organosulphur compounds), which encoded a hypothetical small protein of unknown function. The gene was located upstream of a gene cluster that encodes the components of the sulphate ABC transporter, and it formed a transcriptional unit with sbp, which encoded the periplasmic binding protein of the transporter. The oscA-sbp transcriptional unit was strongly and quickly overexpressed after chromate exposure, suggesting the involvement of oscA in chromate resistance, which was further confirmed by means of a complementation experiment. Phenotype MicroArray (PM) analysis made it possible to assay 1536 phenotypes and also indicated that the oscA gene was involved in the utilization of organosulphur compounds as a sole source of sulphur. This is believed to be the first evidence that oscA plays a role in activating a sulphur starvation response, which is required to cope with oxidative stress induced by chromate." @default.
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- W2020755524 date "2009-01-01" @default.
- W2020755524 modified "2023-10-14" @default.
- W2020755524 title "Involvement of the oscA gene in the sulphur starvation response and in Cr(VI) resistance in Pseudomonas corrugata 28" @default.
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- W2020755524 doi "https://doi.org/10.1099/mic.0.021873-0" @default.
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