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• W2020759312 abstract "Abstract Background : The main mechanism of phototherapy for neonatal hyperbilirubinemia is the production and excretion of (EZ)‐ and (EE)‐cyclobilirubin (4E,15Z‐ and 4E,15E‐cyclobilirubin). Thus, the clinical efficacy of the light source for phototherapy must be evaluated by cyclobilirubin formation from (ZZ)‐bilirubin in in vitro photoirradiation. Methods : In the present study, we investigated the in vitro production pattern of bilirubin photoisomers by phototherapy light from the bilirubin–human serum albumin complex. Results : No clear difference was found in the curves relative to (ZZ)‐bilirubin and its photoisomers under aerobic and anaerobic conditions. The ratio of (EZ)‐cyclobilirubin to (ZZ)‐bilirubin increased proportionately to the dose of irradiating light and no photoequilibrium state was observed analogous to that found in configurational photoisomerization. The concentration of (EZ)‐ and (EE)‐cyclobilirubin increased proportionately with the grade of the percentage decrease in A 460 nm from 0 to 23%. With a percentage decrease in A 460 nm of 23% or more, the cyclobilirubin concentrations reached a steady state. The reason for this appears to be that the concentration of (ZZ)‐bilirubin, a substrate for photoisomers, dropped below 1 mg/100 mL. Biliverdin was produced only in trace amounts. However, the absorption at 520–700 nm increased after a percentage decrease in A 460 nm of more than 23%. Conclusions : The results of the present study show that little bilirubin photooxidation occurred with in vitro aerobic photoirradiation. Before the concentration of cyclobilirubin reaches a steady state, it is theoretically valid to use the percentage decrease in A 460 nm for the evaluation of the clinical efficacy of the light source." @default.
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• W2020759312 date "2001-06-28" @default.
• W2020759312 modified "2023-10-18" @default.
• W2020759312 title "Cyclobilirubin formation by<i>in vitro</i>photoirradiation with neonatal phototherapy light" @default.
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• W2020759312 doi "https://doi.org/10.1046/j.1442-200x.2001.01398.x" @default.
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